PDF(Pubmed)
Abstract：
Avian coccidiosis caused by Eimeria is a serious parasitic disease that poses a threat to the poultry industry. Currently, prevention and treatment mainly rely on the administration of anticoccidials and live oocyst vaccines. However, the prevalence of drug resistance and the inherent limitations of live vaccines have driven the development of novel vaccines. In this study, the surface protein (Et-SAG14), a previously annotated rhoptry protein (Eten5-B), and a gametocyte phosphoglucomutase (Et-PGM1) were characterized and the vaccine potential of the recombinant proteins were evaluated. Et-SAG14 was dispersed in the form of particles in the sporozoite and merozoite stages, whereas Et-PGM1 was distributed in the apical part of the sporozoite and merozoite stages. The previously annotated rhoptry Eten5-B was found not to be located in the rhoptry but distributed in the cytoplasm of sporozoites and merozoites. Immunization with rEten5-B significantly elevated host interferon gamma (IFN-γ) and interleukin 10 (IL-10) transcript levels and exhibited moderate anticoccidial effects with an anticoccidial index (ACI) of 161. Unexpectedly, both recombinant Et-SAG14 and Et-PGM1 immunization significantly reduced host IFN-γ and IL-10 transcription levels, and did not show protection against E. tenella challenge (ACI < 80). These results suggest that the rEten5-B protein can trigger immune protection against E. tenella and may be a potential and effective subunit vaccine for the control of coccidiosis in poultry.
摘要：
由艾美球虫引起的禽球虫病是一种严重的寄生虫病，对养禽业构成威胁。目前,预防和治疗主要依靠抗球虫药和活卵囊疫苗的管理。然而,耐药性的流行和活疫苗的固有局限性推动了新型疫苗的开发。在这项研究中,表面蛋白（Et-SAG14），先前注释的rhoptry蛋白（Eten5-B），和配子体磷酸葡萄糖变位酶（Et-PGM1）进行了表征，并评估了重组蛋白的疫苗潜力。Et-SAG14在子孢子和裂殖子阶段以颗粒形式分散，而Et-PGM1分布于子孢子和裂殖子阶段的顶端。发现先前注释的rhoptryEten5-B不位于rhoptry中，而是分布于子孢子和裂殖子的细胞质中。rEten5-B免疫显着升高宿主干扰素γ（IFN-γ）和白介素10（IL-10）转录水平，并表现出中等的抗球虫作用，抗球虫指数（ACI）为161。出乎意料的是,重组Et-SAG14和Et-PGM1免疫均显着降低宿主IFN-γ和IL-10的转录水平，并且未显示对E.tenella攻击的保护作用（ACI<80）。这些结果表明，rEten5-B蛋白可以触发针对E.tenella的免疫保护，并且可能是控制家禽球虫病的潜在有效的亚单位疫苗。
