PDF(Pubmed)
Abstract：
Dogs often carry methicillin-resistant Staphylococci asymptomatically. These bacteria are frequently linked to conditions such as canine pyoderma and otitis. Close interaction between dogs and humans can facilitate the exchange of resistant strains, particularly Methicillin-resistant Staphylococcus pseudintermedius (MRSP). This represents a public health issue, since these strains, in addition to occasionally causing infections in humans, can also serve as a source of resistance and virulence genes for strains of greater importance in human medicine, such as Staphylococcus aureus. Furthermore, MRSP strains are often multidrug resistant, which ends up compromising the treatment of infections. This study aimed to assess the potential transmission of Staphylococcus pseudintermedius among dogs and their owners. We examined a total of one hundred canine samples collected from cases of pyoderma and otitis to detect the presence of staphylococci. Simultaneously, we conducted evaluations on all dog owners. Staphylococci strains were identified using MALDI-TOF MS and PCR targeting the nuc gene. Methicillin resistance screening was also performed by detecting the mecA gene using PCR. Among the sampled dogs, 64 carried S. pseudintermedius. Nine were identified as MRSP. In six instances, dogs and their owners exhibited S. pseudintermedius. These samples underwent genome sequencing and were screened for antimicrobial resistance genes, SCCmec typing, MLST characterization, and Single Nucleotide Polymorphisms (SNP) analyses. The results of the phylogenetic analysis revealed that in three cases, dogs and owners had closely related isolates, suggesting interspecies transmission. Two of these cases involved MRSP and one MSSP. Moreover, in the two MRSP cases, the same SCCmec type (type V) was detected. Additionally, the sequence type was consistent across all three cases involving dogs and owners (MSSP ST2277, MRSP ST2282, and ST2286). These findings strongly indicate a transmission event. Since Staphylococcus pseudintermedius is primarily isolated from canine samples, it is plausible that dogs may have acted as a potential source. In the remaining three cases, despite identifying the same species in both samples, they had notable phylogenetic differences.
摘要：
狗通常无症状地携带耐甲氧西林葡萄球菌。这些细菌通常与诸如犬脓皮病和耳炎的病症有关。狗与人之间的紧密互动可以促进抗性菌株的交换，特别是耐甲氧西林假中间葡萄球菌（MRSP）。这代表了一个公共卫生问题，由于这些菌株，除了偶尔引起人类感染，还可以作为在人类医学中更重要的菌株的抗性和毒力基因的来源，如金黄色葡萄球菌。此外，MRSP菌株通常具有多重耐药性，最终影响了感染的治疗。这项研究旨在评估假中介葡萄球菌在狗及其主人之间的潜在传播。我们检查了从脓皮病和中耳炎病例中收集的一百个犬样本，以检测葡萄球菌的存在。同时,我们对所有狗主人进行了评估。使用MALDI-TOFMS和靶向nuc基因的PCR鉴定葡萄球菌菌株。还通过使用PCR检测mecA基因进行甲氧西林抗性筛选。在采样的狗中，64载着假中介。9人被确定为MRSP。在六个例子中，狗和它们的主人展示了假中介。这些样本进行了基因组测序，并筛选了抗菌素抗性基因，SCCmec打字,MLST表征,和单核苷酸多态性(SNP)分析。系统发育分析结果显示，在三个案例中，狗和主人有密切相关的分离株，表明种间传播。其中2例涉及MRSP和1例MSSP。此外，在两个MRSP病例中，检测到相同的SCCmec类型(V型)。此外,在涉及犬和主人的3例病例中,序列类型一致(MSSPST2277,MRSPST2282和ST2286).这些发现强烈表明了一个传播事件。由于假中间葡萄球菌主要是从犬类样本中分离出来的,狗可能是潜在的来源，这似乎是合理的。在其余三个案例中，尽管在两个样本中发现了相同的物种，它们有明显的系统发育差异。
