Abstract：
When silica nanoparticles (SiNP) reach the water bodies interact with the already existing pollutants in the environments. This study aimed to evaluate the ecotoxicity of SiNP under the presence/absence of Cu in mosquitofish (Gambusia holbrooki). Fish were exposed to 0, 10 and 100 mg SiNP L-1, alone or mixed with Cu (0.25 mg L-1). After 96 h, the amount of colony forming units (CFU) of bacteria living on the skin mucus was analysed, and oxidative stress, tissue damage enzymes, and neurotoxicity were evaluated. We observed a reduction in CFU when Cu was present in the media. The liver was the target organ, evidencing a decrease in tissue damage enzymatic activities, activation of the antioxidant system in all treatments, and lipid oxidative damage when the SiNP and Cu were mixed. Overall, SiNP ecotoxicity was proved, which could also be enhanced by the presence of ubiquitous elements such as metals.
摘要：
当二氧化硅纳米颗粒（SiNP）到达水体时，与环境中已经存在的污染物相互作用。本研究旨在评估在蚊子（Gambusiaholbrooki）中存在/不存在Cu的情况下SiNP的生态毒性。将鱼单独或与铜（0.25mgL-1）混合暴露于0、10和100mgSiNPL-1。96小时后，分析了生活在皮肤粘液上的细菌的菌落形成单位（CFU）的数量，和氧化应激，组织损伤酶，和神经毒性进行了评估。当Cu存在于介质中时,我们观察到CFU的减少。肝脏是靶器官,证明组织损伤酶活性降低，在所有治疗中激活抗氧化系统，当SiNP和Cu混合时，脂质氧化损伤。总的来说,证明了SiNP的生态毒性，这也可以通过普遍存在的元素如金属的存在来增强。
