UNASSIGNED: Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1.
UNASSIGNED: The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown.
UNASSIGNED: CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN.
■定量PCR和免疫组织化学用于检测EC和正常组织中Ras相关的C3肉毒杆菌毒素底物1(Rac1)和CTTN。分析了这两个基因的表达与预后因素之间的关系。构建CTTN-RNAi慢病毒系统并转染到EC细胞中。通过划痕试验评估迁移和侵袭,Transwell迁移,和入侵检测。通过免疫荧光染色观察到伪足形成。进行Western印迹以检测Rac1的表达。
■在EC组织中Rac1和CTTN的表达水平明显高于正常组织。在EC组中,Rac1与CTTN水平相关。Rac1和CTTN的蛋白表达水平与肌层浸润和分期有关。CTTN击倒后,迁移率,侵入性,EC细胞的迁移能力明显下降。观察到层片随着气泡的出现而消失。CTTN敲低后Rac1卵白表达下降。
■CTTN可能促进层板足虫对EC的入侵和迁移。这种效应可能与CTTN对Rac1的调控有关。