PDF(Pubmed)
Abstract：
UNASSIGNED: Connective tissue subacromial bursa-derived progenitor cells (SBDCs) have been suggested as a potent biologic augment to promote healing of the repaired rotator cuff tendon. Maximizing the amount of retained progenitor cells at the tendon repair site is essential for ensuring an optimal healing environment, warranting a search for proadhesive and proliferative adjuvants. The purpose was to evaluate the effect of magnesium (Mg), platelet-rich plasma (PRP), and a combination of both adjuvants on the in vitro cellular adhesion and proliferation potential of SBDCs on suture material commonly used in rotator cuff surgery.
UNASSIGNED: SBDCs were isolated from subacromial bursa samples harvested during rotator cuff repair and cultured in growth media. Commercially available collagen-coated nonabsorbable flat-braided suture was cut into 1-inch pieces, placed into 48-well culture dishes, and sterilized under ultraviolet light. Either a one-time dose of 5 mM sterile Mg, 0.2 mL of PRP, or a combination of both adjuvants was added, while a group without treatment served as a negative control. Cellular proliferation and adhesion assays on suture material were performed for each treatment condition.
UNASSIGNED: Augmenting the suture with Mg resulted in a significantly increased cellular adhesion (total number of attached cells) of SBDCs compared to PRP alone (31,527 ± 19,884 vs. 13,619 ± 8808; P < .001), no treatment (31,527 ± 19,884 vs. 21,643 ± 8194; P = .016), and combination of both adjuvants (31,527 ± 19,884 vs. 17,121 ± 11,935; P < .001). Further, augmentation with Mg achieved a significant increase in cellular proliferation (absorbance) of SBDCs on suture material when compared to the PRP (0.516 ± 0.207 vs. 0.424 ± 0.131; P = .001) and no treatment (0.516 ± 0.207 vs. 0.383 ± 0.094; P < .001) group. The combination of Mg and PRP showed a significantly higher proliferation potential compared to PRP alone (0.512 ± 0.194 vs. 0.424 ± 0.131; P = .001) and no treatment (0.512 ± 0.194 vs. 0.383 ± 0.094; P < .001). There were no significant differences in the remaining intergroup comparisons (P > .05, respectively).
UNASSIGNED: Augmenting suture material with Mg resulted in a significantly increased cellular adhesion of SBDCs compared to untreated suture material, as well as augmentation with PRP alone or a combination of both adjuvants. Further, Mg with or without PRP augmentation achieved a significant increase in the cellular proliferation of SBDCs on suture material compared to untreated sutures and augmentation with PRP alone. Application of Mg may be a clinically feasible approach to optimizing the use of SBDCs as a biological augment in rotator cuff repair, while combined augmentation with PRP may harness the full potential for optimized tissue recovery due to the high concentration of PRP-derived growth factors.
UNASSIGNED: SBDCs were isolated from subacromial bursa samples harvested during rotator cuff repair and cultured in growth media. Commercially available collagen-coated nonabsorbable flat-braided suture was cut into 1-inch pieces, placed into 48-well culture dishes, and sterilized under ultraviolet light. Either a one-time dose of 5 mM sterile Mg, 0.2 mL of PRP, or a combination of both adjuvants was added, while a group without treatment served as a negative control. Cellular proliferation and adhesion assays on suture material were performed for each treatment condition.
UNASSIGNED: Augmenting the suture with Mg resulted in a significantly increased cellular adhesion (total number of attached cells) of SBDCs compared to PRP alone (31,527 ± 19,884 vs. 13,619 ± 8808; P < .001), no treatment (31,527 ± 19,884 vs. 21,643 ± 8194; P = .016), and combination of both adjuvants (31,527 ± 19,884 vs. 17,121 ± 11,935; P < .001). Further, augmentation with Mg achieved a significant increase in cellular proliferation (absorbance) of SBDCs on suture material when compared to the PRP (0.516 ± 0.207 vs. 0.424 ± 0.131; P = .001) and no treatment (0.516 ± 0.207 vs. 0.383 ± 0.094; P < .001) group. The combination of Mg and PRP showed a significantly higher proliferation potential compared to PRP alone (0.512 ± 0.194 vs. 0.424 ± 0.131; P = .001) and no treatment (0.512 ± 0.194 vs. 0.383 ± 0.094; P < .001). There were no significant differences in the remaining intergroup comparisons (P > .05, respectively).
UNASSIGNED: Augmenting suture material with Mg resulted in a significantly increased cellular adhesion of SBDCs compared to untreated suture material, as well as augmentation with PRP alone or a combination of both adjuvants. Further, Mg with or without PRP augmentation achieved a significant increase in the cellular proliferation of SBDCs on suture material compared to untreated sutures and augmentation with PRP alone. Application of Mg may be a clinically feasible approach to optimizing the use of SBDCs as a biological augment in rotator cuff repair, while combined augmentation with PRP may harness the full potential for optimized tissue recovery due to the high concentration of PRP-derived growth factors.
摘要：
■结缔组织肩峰下囊来源的祖细胞（SBDC）已被认为是一种有效的生物学增强剂，可促进修复的肩袖肌腱的愈合。最大限度地增加肌腱修复部位保留的祖细胞的数量对于确保最佳的愈合环境至关重要。保证寻找前粘附和增殖佐剂。目的是评估镁（Mg）的作用，富血小板血浆(PRP),以及两种佐剂对肩袖手术中常用的缝合材料上SBDC的体外细胞粘附和增殖潜力的组合。
■从肩袖修复期间收获的肩峰下滑囊样品中分离SBDC并在生长培养基中培养。将市售的胶原涂层不可吸收的平编织缝合线切成1英寸的片，放入48孔培养皿中，并在紫外线下消毒。一次剂量的5mM无菌Mg，0.2毫升PRP，或添加两种佐剂的组合，而未治疗的组作为阴性对照。对每种处理条件进行缝合材料上的细胞增殖和粘附测定。
■与单独PRP相比，用Mg增强缝合线导致SBDC的细胞粘附力（附着细胞总数）显着增加（31,527±19,884vs.13,619±8808；P<.001），无治疗（31,527±19,884vs.21,643±8194；P=.016），和两种佐剂的组合(31,527±19,884vs.17,121±11,935；P<.001）。Further,与PRP相比，用Mg增强可以显着增加缝合材料上SBDC的细胞增殖（吸光度）（0.516±0.207vs.0.424±0.131；P=.001）和无治疗（0.516±0.207vs.0.383±0.094；P<.001）组。与单独使用PRP相比，Mg和PRP的组合显示出显着更高的增殖潜力（0.512±0.194vs.0.424±0.131；P=.001）和无治疗（0.512±0.194vs.0.383±0.094;P<.001)。其余组间比较差异均无统计学意义(分别为P>.05)。
■与未处理的缝合材料相比，用Mg增强缝合材料导致SBDC的细胞粘附显着增加，以及单独使用PRP或两种佐剂的组合来增强。Further,与未经处理的缝合线和单独使用PRP的扩增相比,具有或不具有PRP扩增的Mg实现了SBDC在缝合线材料上的细胞增殖的显著增加。Mg的应用可能是临床上可行的方法，以优化SBDC作为肩袖修复中的生物增强剂的使用，由于高浓度的PRP衍生的生长因子，与PRP联合增强可以充分利用优化组织恢复的潜力。
■从肩袖修复期间收获的肩峰下滑囊样品中分离SBDC并在生长培养基中培养。将市售的胶原涂层不可吸收的平编织缝合线切成1英寸的片，放入48孔培养皿中，并在紫外线下消毒。一次剂量的5mM无菌Mg，0.2毫升PRP，或添加两种佐剂的组合，而未治疗的组作为阴性对照。对每种处理条件进行缝合材料上的细胞增殖和粘附测定。
■与单独PRP相比，用Mg增强缝合线导致SBDC的细胞粘附力（附着细胞总数）显着增加（31,527±19,884vs.13,619±8808；P<.001），无治疗（31,527±19,884vs.21,643±8194；P=.016），和两种佐剂的组合(31,527±19,884vs.17,121±11,935；P<.001）。Further,与PRP相比，用Mg增强可以显着增加缝合材料上SBDC的细胞增殖（吸光度）（0.516±0.207vs.0.424±0.131；P=.001）和无治疗（0.516±0.207vs.0.383±0.094；P<.001）组。与单独使用PRP相比，Mg和PRP的组合显示出显着更高的增殖潜力（0.512±0.194vs.0.424±0.131；P=.001）和无治疗（0.512±0.194vs.0.383±0.094;P<.001)。其余组间比较差异均无统计学意义(分别为P>.05)。
■与未处理的缝合材料相比，用Mg增强缝合材料导致SBDC的细胞粘附显着增加，以及单独使用PRP或两种佐剂的组合来增强。Further,与未经处理的缝合线和单独使用PRP的扩增相比,具有或不具有PRP扩增的Mg实现了SBDC在缝合线材料上的细胞增殖的显著增加。Mg的应用可能是临床上可行的方法，以优化SBDC作为肩袖修复中的生物增强剂的使用，由于高浓度的PRP衍生的生长因子，与PRP联合增强可以充分利用优化组织恢复的潜力。
