PDF(Pubmed)
Abstract：
The current study was designed to evaluate the effect of sequential low and high dietary linseed oil (LO; as omega-3 enriched fatty acid; FA) before and post insemination, respectively, on different plasma variables of ewes. Fat-tailed Qezel ewes were assigned randomly to be fed a diet enriched with 3% LO (n = 30) or the saturated FA (SFA; n = 30) three weeks before insemination (Day 0). The lipogenic diet supplemented with 6% LO or SFA was fed after insemination until Day +21. The control ewes were fed an isocaloric and isonitrogenous diet with no additional FA during the study. Estrus was synchronized by inserting a vaginal sponge (Spongavet®) for 12 days + 500 IU equine chorionic gonadotropin (eCG; Gonaser®), and ewes were inseminated via laparoscopic approach 56-59 h after eCG injection. The size of ovarian structures was assessed by transvaginal ultrasonography at -21, -14, -2, 0, and +10 days. Blood samples were collected weekly to measure the plasma\'s different biochemical variables and FA profile. Treatment did not affect the amounts of glucose, aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin-10, interleukin-2, and non-esterified FA (p > 0.05). Conversely, concentrations of triglyceride, cholesterol, tumor necrosis factor-alpha, and insulin-like growth factor-1 were higher in SFA-fed ewes relative to control animals (p < 0.05). LO feeding resulted in greater amounts of n-3 FA isomers in plasma, while higher amounts of stearic acid were detected in SFA fed group 0 and +21 (p < 0.05). The number of ovarian follicles and corpora lutea also were not affected by treatment. Other reproductive variables were not affected by treatment except for the reproductive rate. It seems that LO or SFA feeding of fat-tailed ewes peri-insemination period was not superior to the isocaloric non-additional fat diet provided for the control group during the non-breeding season.
摘要：
本研究旨在评估在授精前后连续低饮食和高饮食亚麻籽油（LO；作为富含omega-3的脂肪酸；FA）的效果。分别,关于母羊不同的血浆变量。在授精前三周（第0天），随机分配肥尾Qezel母羊饲喂富含3％LO（n=30）或饱和FA（SFA；n=30）的饮食。在授精后喂养补充有6%LO或SFA的生脂饮食直至第+21天。在研究过程中，对对照母羊饲喂等热量和等氮的饮食，没有额外的FA。通过插入阴道海绵（Spongavet®）12天+500IU马绒毛膜促性腺激素（eCG;Gonaser®）来同步发情，eCG注射后56-59小时，通过腹腔镜方法对母羊进行授精。在-21、-14、-2、0和+10天经阴道超声检查评估卵巢结构的大小。每周收集血样以测量血浆的不同生化变量和FA谱。治疗不影响葡萄糖的量，天冬氨酸转氨酶,丙氨酸氨基转移酶，乳酸脱氢酶,白细胞介素-10、白细胞介素-2和非酯化FA(p>0.05)。相反，甘油三酯的浓度，胆固醇,肿瘤坏死因子-α,和胰岛素样生长因子-1在SFA喂养的母羊中相对于对照动物更高(p<0.05)。LO进料导致血浆中更大量的n-3FA异构体，而SFA饲喂0组和+21组的硬脂酸含量较高(p<0.05)。卵巢卵泡和黄体的数量也不受治疗的影响。除繁殖率外，其他生殖变量均不受治疗影响。似乎在非繁殖季节，对脂尾母羊围授精期的LO或SFA喂养并不优于对照组提供的等热量非额外脂肪饮食。
