PDF(Pubmed)
Abstract：
OBJECTIVE: Burkholderia cenocepacia causes severe infections in cystic fibrosis (CF) patients. CF patients are prone to reoccurring infections due to the accumulation of mucus in their lungs, where bacteria can adhere and grow. Some of the antibiotics that inhibit B. cenocepacia in the laboratory are not effective for CF patients. A major contributor to poor clinical outcomes is that antibiotic testing in laboratories occurs under conditions that are different from those of sputum. CF sputum may be acidic and have increased concentrations of iron and zinc. Here, we used a medium that mimics CF sputum and found that acidic pH decreased the activity of many of the antibiotics used against B. cenocepacia. In addition, we assessed susceptibility to more than 500 antibiotics and found four active compounds against B. cenocepacia. Our findings give a better understanding of the lack of a relationship between susceptibility testing and the clinical outcome when treating B. cenocepacia infections.
摘要：
目的：伯克霍尔德氏菌在囊性纤维化（CF）患者中引起严重感染。CF患者由于肺部粘液的积聚而容易发生感染，细菌可以粘附和生长的地方。在实验室中抑制头孢双歧杆菌的一些抗生素对CF患者无效。临床结果不佳的主要原因是实验室中的抗生素测试发生在不同于痰的条件下。CF痰可能是酸性的，并且铁和锌的浓度增加。这里,我们使用了一种模拟CF痰液的培养基，发现酸性pH降低了许多抗生素的活性。此外,我们评估了超过500种抗生素的敏感性,并发现了4种活性化合物对头孢双歧杆菌。我们的发现使人们更好地了解了治疗隐血芽孢杆菌感染时药敏试验与临床结果之间缺乏关系。
