UNASSIGNED: In the present study, disease and growth indices were compared between grafted and original wax gourds after infection with Fusarium. High level of disease resistance was observed in the grafted wax gourd, with a lower disease index and low impacts on growth after infection. RNA-seq was performed to identify the differentially expressed genes (DEGs) between the adjacent treatment time points in the grafted and original wax gourds, respectively. Then a comparative temporal analysis was performed and a total of 1,190 genes were identified to show different gene expression patterns between the two wax gourd groups during Fusarium infection.
UNASSIGNED: Here, high level of disease resistance was observed in the grafted wax gourd, with a lower disease index and low impacts on growth after infection. The DEG number was higher in grafted group than original group, and the enriched functional categories and pathways of DEGs were largely inconsistent between the two groups. These genes were enriched in multiple pathways, of which the mitogen-activated protein kinase (MAPK) signaling pathway enhanced the early defense response, and cutin, suberin, and wax biosynthesis signaling pathways enhanced surface resistance in grafted wax gourd in comparison to original group. Our study provides insights into the gene regulatory mechanisms underlying the resistance of grafted wax gourds to Fusarium wilt infection, which will facilitate the breeding and production of wilt-resistant rootstocks.
■在本研究中,比较了镰刀菌感染后嫁接和原始冬瓜的疾病和生长指标。在嫁接的冬瓜中观察到高水平的抗病性,疾病指数较低,感染后对生长的影响较低。进行RNA-seq以鉴定嫁接和原始冬瓜中相邻处理时间点之间的差异表达基因(DEG),分别。然后进行了比较时间分析,鉴定出总共1190个基因,在镰刀菌感染过程中,两个冬瓜组之间显示出不同的基因表达模式。
■这里,在嫁接的冬瓜中观察到高水平的抗病性,疾病指数较低,感染后对生长的影响较低。嫁接组的DEG数高于原始组,两组之间DEGs的丰富功能类别和途径在很大程度上不一致。这些基因富含多种途径,其中丝裂原活化蛋白激酶(MAPK)信号通路增强了早期防御反应,还有角质,suberin,与原始组相比,嫁接冬瓜的表面抗性和蜡生物合成信号通路增强。我们的研究提供了有关嫁接冬瓜对枯萎病感染的抗性的基因调控机制的见解,这将有利于抗枯萎病砧木的育种和生产。