Abstract：
Circular RNAs (circRNAs) regulate the function of vascular smooth muscle cells (VSMCs) in atherosclerosis (AS) progression. We aimed to explore the role of circUSP9X in oxidized low-density lipoprotein (ox-LDL)-induced VSMCs. Cell proliferation was assessed using cell counting kit-8 and EDU assays. Cell migration was evaluated using Transwell and wound healing assays. The interaction between circUSP9X or STIM1 and miR-599 was analyzed using dual-luciferase reporter and RNA pull-down assays. Their levels were examined using quantitative real-time PCR. CircUSP9X and STIM1 expression was increased, whereas miR-599 expression was reduced in the serum of patients with AS and ox-LDL-stimulated VSMCs. Overexpression of circUSP9X facilitated the proliferation and migration of VSMCs induced by ox-LDL. CircUSP9X sponged miR-599, which targeted STIM1. MiR-599 reversed the effects induced by circUSP9X, and STIM1 reversed the effects induced by miR-599. Taken together, CircUSP9X promoted proliferation and migration in ox-LDL-treated VSMCs via the miR-599/STIM1 axis, providing a theoretical basis for the role of circUSP9X/miR-599/STIM1 axis in AS.
摘要：
环状RNA（circularRNAs）在动脉粥样硬化（AS）进展中调节血管平滑肌细胞（VSMC）的功能。我们旨在探讨circUSP9X在氧化低密度脂蛋白（ox-LDL）诱导的VSMC中的作用。使用细胞计数试剂盒-8和EDU测定评估细胞增殖。使用Transwell和伤口愈合测定评估细胞迁移。使用双荧光素酶报告基因和RNA下拉测定分析circUSP9X或STIM1与miR-599之间的相互作用。使用定量实时PCR检查它们的水平。CircUSP9X和STIM1表达增加，而miR-599在AS和ox-LDL刺激的VSMC患者血清中的表达降低。circusp9X的过表达促进ox-LDL诱导的VSMC的增殖和迁移。CircUSP9X海绵化了靶向STIM1的miR-599。MiR-599逆转了circUSP9X引起的效应，和STIM1逆转了miR-599诱导的效应。一起来看,CircUSP9X通过miR-599/STIM1轴促进ox-LDL处理的VSMC的增殖和迁移，为circUSP9X/miR-599/STIM1轴在AS中的作用提供理论依据。
