PDF(Pubmed)
Abstract：
Bacterial canker caused by Pseudomonas syringae pv. syringae (Pss) is responsible for substantial loss to the production of sweet cherry in Chile. To date, the molecular mechanisms of the Pss-sweet cherry interaction and the disease-related genes in the plant are poorly understood. In order to gain insight into these aspects, a transcriptomic analysis of the sweet cherry cultivar \'Lapins\' for differentially expressed genes (DEGs) in response to Pss inoculation was conducted. Three Pss strains, A1M3, A1M197, and 11116_b1, were inoculated in young twigs, and RNA was extracted from tissue samples at the inoculation site and distal sections. RNA sequencing and transcriptomic expression analysis revealed that the three strains induced different patterns of responses in local and distal tissues. In the local tissues, A1M3 triggered a much more extensive response than the other two strains, enriching DEGs especially involved in photosynthesis. In the distal tissues, the three strains triggered a comparable extent of responses, among which 11116_b1 induced a group of DEGs involved in defense responses. Furthermore, tissues from various inoculations exhibited an enrichment of DEGs related to carbohydrate metabolism, terpene metabolism, and cell wall biogenesis. This study opened doors to future research on the Pss-sweet cherry interaction, immunity responses, and disease control.
摘要：
丁香假单胞菌pv引起的细菌性溃疡。丁香科（Pss）是智利甜樱桃生产的重大损失。迄今为止,植物中Pss-甜樱桃相互作用和疾病相关基因的分子机制知之甚少。为了深入了解这些方面，对响应Pss接种的甜樱桃品种“Lapins”的差异表达基因（DEGs）进行了转录组学分析。三个Pss菌株，将A1M3，A1M197和11116_b1接种在幼枝中，从接种部位和远端切片的组织样品中提取RNA。RNA测序和转录组表达分析显示，这三种菌株在局部和远端组织中诱导了不同的反应模式。在局部组织中，A1M3引发了比其他两种菌株更广泛的反应，富集特别参与光合作用的DEGs。在远端组织中，这三种菌株引发了相当程度的反应，其中11116_b1诱导了一组参与防御反应的DEG。此外，来自各种接种的组织表现出与碳水化合物代谢相关的DEGs的富集，萜烯代谢，和细胞壁生物发生。这项研究为Pss-甜樱桃相互作用的未来研究打开了大门，免疫反应，和疾病控制。
