PDF(Pubmed)
Abstract：
Gut microbiota has been described as a new \'organ\' that interferes with host physiology by its metabolites produced from the utilization and biotransformation of undigested food components. Fu Ling (FL), the sclerotia of fungi Wolfiporia cocos, contains β-glucan, which is a known natural polysaccharide with strong medicinal efficacy. This study endeavors to evaluate the fermentability of FL and polysaccharides extracted from its sclerotia. An in vitro fermentation of structurally characterized FL and its β-glucan by human fecal microbiota was conducted. Total bacterial count, pH change, short-chain fatty acid profile and microbiota profile were assessed post-fermentation. FL containing over 70% of β-(1 → 3) and (1 → 6)-glucans with a low degree of branching of 0.24 could enhance acetic acid (a major microbial metabolite) production. Both FL and its extracted β-glucan had similar modulation on microbial composition. They enriched Phascolarctobacterium faecium, Bacteroides dorei and Parabacteroides distasonis, all of which are shown to possess anti-inflammatory effects. FL polysaccharide can be utilized as a natural whole food for its potential health benefits to human gut bacteria.
摘要：
肠道微生物群被描述为一种新的“器官”，通过利用和生物转化未消化的食物成分而产生的代谢产物来干扰宿主的生理。傅玲（FL），真菌Wolfaporiacos的菌核，含有β-葡聚糖，这是一种已知的天然多糖，具有很强的药用功效。本研究试图评估FL和从其菌核中提取的多糖的发酵能力。通过人粪便微生物群进行结构表征的FL及其β-葡聚糖的体外发酵。细菌总数,pH值变化,发酵后评估短链脂肪酸谱和微生物区系谱。含有70％以上的β-（1→3）和（1→6）-葡聚糖的FL，支化度低为0.24，可以增强乙酸（一种主要的微生物代谢产物）的产生。FL及其提取的β-葡聚糖对微生物组成具有相似的调节。它们富集了屎相弯曲杆菌,多氏杆菌属和双杆菌属，所有这些都被证明具有抗炎作用。FL多糖由于其对人体肠道细菌的潜在健康益处而可用作天然整体食品。
