PDF(Pubmed)
Abstract：
Skin microbiome dysbiosis has deleterious effects, and the factors influencing burn scar formation, which affects the scar microbiome composition, are unknown. Therefore, we investigated the effects of various factors influencing scar formation on the scar microbiome composition in patients with burns. We collected samples from the burn scar center and margin of 40 patients with burns, subgrouped by factors influencing scar formation. Scar microbiome composition-influencing factors were analyzed using univariate and multivariate analyses. Skin graft, hospitalization period, intensive care unit (ICU) admission, burn degree, sex, age, total body surface area burned (TBSA), time post-injury, transepidermal water loss, the erythrocyte sedimentation rate, and C-reactive protein levels were identified as factors influencing burn scar microbiome composition. Only TBSA and ICU admission were associated with significant differences in alpha diversity. Alpha diversity significantly decreased with an increase in TBSA and was significantly lower in patients admitted to the ICU than in those not admitted to the ICU. Furthermore, we identified microorganisms associated with various explanatory variables. Our cross-sectional systems biology study confirmed that various variables influence the scar microbiome composition in patients with burns, each of which is associated with various microorganisms. Therefore, these factors should be considered during the application of skin microbiota for burn scar management.
摘要：
皮肤微生物群失调具有有害影响，以及影响烧伤瘢痕形成的因素，这会影响疤痕微生物组的组成，是未知的。因此,我们研究了各种影响瘢痕形成的因素对烧伤患者瘢痕菌群组成的影响。我们从40例烧伤患者的烧伤瘢痕中心和边缘收集样本，按影响瘢痕形成的因素分组。采用单因素和多因素分析方法对瘢痕菌群组成影响因素进行分析。皮肤移植,住院期间，重症监护病房(ICU)入院,烧伤程度,性别,年龄,烧伤总表面积（TBSA），受伤后的时间，经皮水分流失，红细胞沉降率，和C反应蛋白水平被确定为影响烧伤瘢痕微生物组组成的因素。只有TBSA和ICU入院与α多样性的显着差异相关。随着TBSA的增加，α多样性显着降低，并且在ICU住院的患者中显着低于未ICU住院的患者。此外，我们确定了与各种解释变量相关的微生物。我们的横断面系统生物学研究证实，各种变量会影响烧伤患者的瘢痕微生物组组成，每个都与各种微生物有关。因此,在应用皮肤微生物群治疗烧伤瘢痕时，应考虑这些因素。
