PDF(Pubmed)
Abstract：
Fish speciation was accompanied by changes in the urogenital system anatomy. In evolutionarily modern Teleostei, male reproductive tracts are fully separated from the excretory system, while in evolutionarily ancient Chondrostei and Holostei, the excretory and reproductive tracts are not separated. Sturgeon post-testicular sperm maturation (PTSM) occurring as a result of sperm/urine mixing is phenomenologically well described, while, in holosteans, functional intimacy of seminal ducts with kidney ducts and the existence of PTSM still need to be addressed. In Lepisosteus platostomus (Holostei), sperm samples were collected from testes (TS), efferent ducts (EDS), and Wolffian ducts (WDS). While WDS was motile, no motility was found in TS and EDS. The existence of PTSM was checked by in vitro PTSM procedure. After TS and EDS incubation in seminal fluid from WDS, no more than 5% motile spermatozoa were observed in TS, whereas in EDS the motility percentage was up to 75%. Experimental dyeing of urogenital ducts in gars and sturgeons revealed some differences in the interconnection between sperm ducts and kidneys. It is concluded that post-testicular sperm maturation occurs in gars and suggests that infraclass Holostei occupies an intermediate evolutionary position between Teleostei and Chondrostei in the anatomical arrangement of the urogenital system.
摘要：
鱼类物种形成伴随着泌尿生殖系统解剖结构的变化。在进化的现代Teleostei中，男性生殖道与排泄系统完全分离，在进化上古老的Chondrostei和Holostei中，排泄物和生殖道没有分开。在现象学上很好地描述了由于精子/尿液混合而发生的the鱼睾丸后精子成熟（PTSM），while,在Holosteans中，精管与肾管的功能亲密关系以及PTSM的存在仍需解决。在Lepisosteusplatostomus(Holostei)中，从睾丸（TS）收集精子样本，输出管(EDS),和沃尔夫导管（WDS）。虽然WDS是活跃的，在TS和EDS中未发现运动性。通过体外PTSM程序检查PTSM的存在。在来自WDS的精液中进行TS和EDS孵育后，在TS中观察到不超过5％的活动精子，而在EDS中，运动百分比高达75％。对鳞茎和st鱼的泌尿生殖道进行实验染色显示，精子管和肾脏之间的相互连接存在一些差异。结论是睾丸后精子成熟发生在gar中，这表明infracclassHolostei在泌尿生殖系统的解剖排列中占据了Teleostei和Chondrostei之间的中间进化位置。
