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Abstract：
Background Natural killer cells (NK cells) are important mediators of innate immune regulation and literature has shown that they have a role in shaping the adaptive immune system. Objective The present study was undertaken to analyze the NK cell count in systemic lupus erythematosus (SLE) patients as compared to that of controls. Materials and methods Safety of Estrogens in Lupus Erythematosus National Assessment SLE Disease Activity Index (SELENA-SLEDAI) score was assessed in 32 SLE cases. CD3(-) cells were identified as NK cells on flow cytometry, and then their subsets CD56(+) and CD16(+) cells were identified compared to 30 healthy controls. Receiver Operating Characteristic (ROC) curve analysis was performed on NK cells to attempt to determine a cut-off point. Results The CD3(-) NK cells, including the percentages of CD56(+) and CD16(+), were significantly (p<0.001) reduced in SLE patients (12.35%, and 18.7%) as compared to controls (24.67%, and 46.6%). On ROC curve analysis, cut-off values <481/cumm with sensitivity of 86.7% and specificity of 84.4% for CD3(-) NK cells (p<0.001), <23% with 60% sensitivity and 75% specificity for CD56(+) NK cells (p<0.001), and <29% with sensitivity of 70% and specificity of 87.5% for CD16(+) NK cells (p<0.001) were noted. Subsets of NK cells showed no association with the clinicopathological parameters like age, sex, disease activity, anti-nuclear antibodies (ANA), dsDNA, absolute lymphocyte count, and renal involvement. Conclusion NK cells, and their subpopulations of CD56(+) and CD16(+) cells, are decreased in patients with SLE as compared to controls.
摘要：
背景技术自然杀伤细胞(NK细胞)是先天免疫调节的重要介质,文献表明它们在形成适应性免疫系统中起作用。目的分析系统性红斑狼疮（SLE）患者与对照组的NK细胞计数。材料和方法在32例SLE病例中评估了红斑狼疮国家评估SLE疾病活动指数（SELENA-SLEDAI）评分中雌激素的安全性。流式细胞术将CD3(-)细胞鉴定为NK细胞,然后将它们的亚群CD56(+)和CD16(+)细胞与30个健康对照进行比较。对NK细胞进行受试者工作特征(ROC)曲线分析以试图确定截止点。结果CD3(-)NK细胞,包括CD56(+)和CD16(+)的百分比，在SLE患者中显著(p<0.001)降低(12.35%，和18.7%)，与对照组(24.67%，和46.6%）。在ROC曲线分析上,截断值<481/cumm，对CD3（-）NK细胞的敏感性为86.7％，特异性为84.4％（p<0.001），<23％，对CD56（+）NK细胞具有60％的敏感性和75％的特异性（p<0.001），和<29%,对于CD16(+)NK细胞具有70%的敏感性和87.5%的特异性(p<0.001)。NK细胞亚群与临床病理参数如年龄无关，性别,疾病活动,抗核抗体（ANA），dsDNA,绝对淋巴细胞计数，和肾脏受累。结论NK细胞,以及它们的CD56(+)和CD16(+)细胞亚群，与对照组相比，SLE患者的发病率下降。
