Abstract：
The increasing occurrence of harmful algal blooms, mostly of the dinoflagellate Alexandrium catenella in Canada, profoundly disrupts mussel aquaculture. These filter-feeding shellfish feed on A. catenella and accumulate paralytic shellfish toxins, such as saxitoxin, in tissues, making them unsafe for human consumption. Algal toxins also have detrimental effects upon several physiological functions in mussels, but particularly on the activity of hemocytes - the mussel immune cells. The objective of this work was to determine the effects of experimental exposure to A. catenella upon hemocyte metabolism and activity in the blue mussel, Mytilus edulis. To do so, mussels were exposed to cultures of the toxic dinoflagellate A. catenella for 120 h. The resulting mussel saxitoxin load had measurable effects upon survival of hemocytes and induced a stress response measured as increased ROS production. The neutral lipid fraction of mussel hemocytes decreased two-fold, suggesting a differential use of lipids. Metabolomic 1H nuclear magnetic resonance (NMR) analysis showed that A. catenella modified the energy metabolism of hemocytes as well as hemocyte osmolyte composition. The modified energy metabolism was reenforced by contrasting plasma metabolomes between control and exposed mussels, suggesting that the blue mussel may reduce feed assimilation when exposed to A. catenella.
摘要：
有害藻华的增加，大部分是加拿大的甲藻藻亚历山大菌，严重破坏了贻贝水产养殖。这些滤食贝类以A.catenella为食，并积累麻痹性贝类毒素，比如沙克毒素,在组织中，使它们不适合人类消费。藻类毒素也对贻贝的几种生理功能产生有害影响，但特别是血细胞的活性-贻贝免疫细胞。这项工作的目的是确定实验暴露于A.catenella对蓝色贻贝血细胞代谢和活性的影响，Mytilusedulis.要做到这一点，将贻贝暴露于有毒的鞭毛藻A.catenella的培养物中120小时。所得的贻贝毒素负荷对血细胞的存活具有可测量的影响，并诱导了应激反应，测量为增加的ROS产生。贻贝血细胞的中性脂质部分减少了两倍，表明脂质的不同用途。代谢组学1H核磁共振（NMR）分析表明，A.catenella改变了血细胞的能量代谢以及血细胞渗透压组成。通过对比对照和暴露的贻贝之间的血浆代谢组来增强改变的能量代谢，表明蓝贻贝在暴露于A.catenella时可能会减少饲料同化。
