PDF(Pubmed)
Abstract：
Red blood cells (RBC) from patients with sickle cell disease (SCD) have elevated calcium levels at baseline, which are further elevated upon deoxygenation. Here we examined baseline calcium levels and calcium flux in RBCs from a mouse model of SCD mice. We found that akin to humans with SCD, sickle (HbSS) Townes mice, have higher baseline levels and increased calcium flux in RBCs compared to control (HbAA) animals. As HbSS mice, unlike humans with SCD, have high mean corpuscular volume compared with HbAA, we highlight the importance of adjusting biochemical results to number of RBCs rather than hematocrit during the analysis and interpretation of the results. Our findings add to the face validity of humanized sickle cell mice and support its use for studies of RBC calcium flux in SCD.
摘要：
镰状细胞病（SCD）患者的红细胞（RBC）在基线时钙水平升高，在脱氧时进一步升高。在这里，我们检查了SCD小鼠模型的RBC中的基线钙水平和钙通量。我们发现类似于患有SCD的人类，镰刀（HbSS）汤斯老鼠，与对照(HbAA)动物相比,在红细胞中具有更高的基线水平和增加的钙通量。作为HbSS小鼠，与患有SCD的人类不同，与HbAA相比，平均红细胞体积较高，在分析和解释结果时,我们强调了根据红细胞数量而非血细胞比容调整生化结果的重要性.我们的发现增加了人源化镰状细胞小鼠的表面有效性，并支持将其用于SCD中RBC钙通量的研究。
