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Abstract：
OBJECTIVE: Jining Grey goat is a local Chinese goat breed that is well known for its high fertility and excellent meat quality but shows low meat production performance. Numerous studies have focused on revealing the genetic mechanism of its high fertility, but its highlighting meat quality and muscle growth mechanism still need to be studied.
METHODS: In this research, an integrative analysis of the genomics and transcriptomics of Jining Grey goats compared with Boer goats was performed to identify candidate genes and pathways related to the mechanisms of meat quality and muscle development.
RESULTS: Our results overlap among five genes (ABHD2, FN1, PGM2L1, PRKAG3, RAVER2) and detected a set of candidate genes associated with fatty acid metabolism (PRKAG3, HADHB, FASN, ACADM), amino acid metabolism (KMT2C, PLOD3, NSD2, SETDB1, STT3B, MAN1A2, BCKDHB, NAT8L, P4HA3) and muscle development (MSTN, PPARGC1A, ANKRD2). Several pathways have also been detected, such as the FoxO signaling pathway and Apelin signaling pathway that play roles in lipid metabolism, lysine degradation, N-glycan biosynthesis, valine, leucine and isoleucine degradation that involving with amino acid metabolism.
CONCLUSIONS: The comparative genomic and transcriptomic analysis of Jining Grey goat and Boer goat revealed the mechanisms underlying the meat quality and meat productive performance of goats. These results provide valuable information for future breeding of goats.
METHODS: In this research, an integrative analysis of the genomics and transcriptomics of Jining Grey goats compared with Boer goats was performed to identify candidate genes and pathways related to the mechanisms of meat quality and muscle development.
RESULTS: Our results overlap among five genes (ABHD2, FN1, PGM2L1, PRKAG3, RAVER2) and detected a set of candidate genes associated with fatty acid metabolism (PRKAG3, HADHB, FASN, ACADM), amino acid metabolism (KMT2C, PLOD3, NSD2, SETDB1, STT3B, MAN1A2, BCKDHB, NAT8L, P4HA3) and muscle development (MSTN, PPARGC1A, ANKRD2). Several pathways have also been detected, such as the FoxO signaling pathway and Apelin signaling pathway that play roles in lipid metabolism, lysine degradation, N-glycan biosynthesis, valine, leucine and isoleucine degradation that involving with amino acid metabolism.
CONCLUSIONS: The comparative genomic and transcriptomic analysis of Jining Grey goat and Boer goat revealed the mechanisms underlying the meat quality and meat productive performance of goats. These results provide valuable information for future breeding of goats.
摘要：
目的：济宁灰山羊是中国本地山羊品种，以其高生育力和优良的肉质而闻名，但表现出较低的产肉性能。众多研究都集中在揭示其高生育力的遗传机制,但其突出的肉质和肌肉生长机制仍需研究。
方法：在这项研究中，对济宁灰山羊与波尔山羊的基因组学和转录组学进行了综合分析，以鉴定与肉质和肌肉发育机制相关的候选基因和途径。
结果：我们的结果在五个基因（ABHD2，FN1，PGM2L1，PRKAG3，RAVER2）中重叠，并检测到一组与脂肪酸代谢相关的候选基因（PRKAG3，HADHB，FASN,ACADM)，氨基酸代谢(KMT2C，PLOD3,NSD2,SETDB1,STT3B,MAN1A2,BCKDHB,NAT8L,P4HA3）和肌肉发育（MSTN，PPARGC1A,ANKRD2).还发现了几种途径，如在脂质代谢中起作用的FoxO信号通路和Apelin信号通路,赖氨酸降解，N-聚糖生物合成，缬氨酸,涉及氨基酸代谢的亮氨酸和异亮氨酸降解。
结论：对济宁灰山羊和波尔山羊的比较基因组和转录组学分析揭示了山羊肉品质和肉生产性能的潜在机制。这些结果为今后山羊育种提供了有价值的信息。
方法：在这项研究中，对济宁灰山羊与波尔山羊的基因组学和转录组学进行了综合分析，以鉴定与肉质和肌肉发育机制相关的候选基因和途径。
结果：我们的结果在五个基因（ABHD2，FN1，PGM2L1，PRKAG3，RAVER2）中重叠，并检测到一组与脂肪酸代谢相关的候选基因（PRKAG3，HADHB，FASN,ACADM)，氨基酸代谢(KMT2C，PLOD3,NSD2,SETDB1,STT3B,MAN1A2,BCKDHB,NAT8L,P4HA3）和肌肉发育（MSTN，PPARGC1A,ANKRD2).还发现了几种途径，如在脂质代谢中起作用的FoxO信号通路和Apelin信号通路,赖氨酸降解，N-聚糖生物合成，缬氨酸,涉及氨基酸代谢的亮氨酸和异亮氨酸降解。
结论：对济宁灰山羊和波尔山羊的比较基因组和转录组学分析揭示了山羊肉品质和肉生产性能的潜在机制。这些结果为今后山羊育种提供了有价值的信息。
