Abstract：
The fall armyworm, Spodoptera frugiperda, is a notorious polyphagous pest that causes serious economic losses in crucial crops and has invaded Africa and Asia. Lufenuron is widely used for controlling S. frugiperda in China, owing to its high toxicity against this key pest, and less pollution and little impact on natural enemies. In the present study, the sublethal and transgenerational effects of lufenuron on S. frugiperda were investigated to provide in-depth information for the rational use of lufenuron. Results showed that the development time and pupae weight were not significantly affected following exposure of females to LC10 and LC25 and male S. frugiperda to the LC10 of lufenuron. However, LC25 exposure significantly reduced pupal and total development time and pupae weight of male S. frugiperda. The longevity of S. frugiperda adults was prolonged by lufenuron and the fecundity of S. frugiperda treated with LC10 of lufenuron was significantly increased by 40% compared to the control. In addition, our study demonstrated that the LC25 of lufenuron had transgenerational effects on the progeny generation. The development time of female S. frugiperda whose parents were exposed to LC25 of lufenuron was significantly decreased compared to the control. And then, the expression profiles of Vg, VgR, JHEH, JHE, JHAMT, JHBP, CYP307A1, CYP306A1, CYP302A1 and CYP314A1 genes involved in insect reproduction and development were analyzed using Quantitative Real-Time PCR (RT-qPCR). Results showed that Vg, VgR, JHE, JHAMT, and CYP306A1 were significantly upregulated at the LC10 of lufenuron, which revealed that these upregulated genes might be linked with increased fecundity of S. frugiperda. Taken together, these findings highlighted the importance of sublethal and transgenerational effects under laboratory conditions and these effects may change the population dynamics in the field. Therefore, our study provided valuable information for promoting the rational use of lufenuron for controlling S. frugiperda.
摘要：
秋天的粘虫，节食夜蛾,是一种臭名昭著的多食性害虫，在关键作物中造成严重的经济损失，并入侵了非洲和亚洲。Lufenuron在中国被广泛用于控制S.frugiperda，由于它对这种关键害虫的高毒性，污染少，对天敌的影响小。在本研究中,本研究调查了氯芬隆对S.frugiperda的亚致死效应和跨代效应,为氯芬隆的合理使用提供了深入的信息.结果表明，雌性暴露于LC10和LC25以及雄性S.frugiperda暴露于LC10后，发育时间和p的重量没有显着影响。然而,LC25暴露显着降低了雄性S.frugiperda的p，总发育时间和p的重量。鲁芬隆延长了S.frugiperda成虫的寿命，并且与对照相比，用LC10的鲁芬隆处理的S.frugiperda的繁殖力显着增加了40％。此外,我们的研究表明，氯芬隆的LC25对后代有跨代效应。与对照组相比，父母暴露于LC25的雌性S.frugiperda的发育时间显着减少。然后,Vg的表达谱，VgR,JHEH,JHE,JHAMT,JHBP,使用定量实时PCR（RT-qPCR）分析了与昆虫繁殖和发育有关的CYP307A1，CYP306A1，CYP302A1和CYP314A1基因。结果表明,Vg,VgR,JHE,JHAMT,CYP306A1在LC10处显著上调，这表明这些上调的基因可能与S.frugiperda的繁殖力增加有关。一起来看,这些发现强调了实验室条件下亚致死效应和跨代效应的重要性,这些效应可能会改变该领域的种群动态.因此,我们的研究为促进氯芬隆的合理使用控制水果链球菌提供了有价值的信息。
