Abstract：
OBJECTIVE: This study aimed to develop an anti-aging nanoformulation with Curcuma heyneana extract as bioactive substance.
BACKGROUND: Curcuma heyneana Valeton & Zipj extract has been proven in previous research to have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need to penetrate into deeper skin layers to ensure effectivity. Thus, a transdermal delivery system is needed to deliver the extract to a deeper skin layer.
OBJECTIVE: The objective of the study was to compare the permeability and anti-ageing activity of liposomal and ethosomal formulations of C. heynena rhizome ethanolic extract.
METHODS: In this study, C. heyneana extract was loaded into a phospholipid vesicular system in the form of liposome and ethosome formulations using the ethanolic injection method. The anti-ageing activity was assessed by analyzing the epidermal thickness, number of sunburn cells, distance between collagen fibres, and number of fibroblasts. While the histologic specimen scoring was carried out for the in vivo penetration study.
RESULTS: The ethosomal formulation had been found to have better penetration ability since it was able to reach the lower dermis area compared to the liposomes, which only reached the upper dermis. The ethosomal formulation of C. heyneana extract exhibited a better anti-ageing activity based on the parameters of epidermal thickness, sunburn cell count, fibroblast count, and the distance between collagen fibres in rat skin histology.
CONCLUSIONS: Ethosomes have been found to be a more proficient carrier system for transdermal delivery of C. heyneana extract compared to liposomes. Meanwhile, their penetration correlated with the effectivity of the formulation, suggesting that the vesicular system enhanced the penetration ability of the extract.
BACKGROUND: Curcuma heyneana Valeton & Zipj extract has been proven in previous research to have antioxidant, anti-ageing, anti-inflammatory, and wound healing properties, which makes it a potential bioactive material for anti-ageing and sunscreen cosmetic products. Phytoantioxidants need to penetrate into deeper skin layers to ensure effectivity. Thus, a transdermal delivery system is needed to deliver the extract to a deeper skin layer.
OBJECTIVE: The objective of the study was to compare the permeability and anti-ageing activity of liposomal and ethosomal formulations of C. heynena rhizome ethanolic extract.
METHODS: In this study, C. heyneana extract was loaded into a phospholipid vesicular system in the form of liposome and ethosome formulations using the ethanolic injection method. The anti-ageing activity was assessed by analyzing the epidermal thickness, number of sunburn cells, distance between collagen fibres, and number of fibroblasts. While the histologic specimen scoring was carried out for the in vivo penetration study.
RESULTS: The ethosomal formulation had been found to have better penetration ability since it was able to reach the lower dermis area compared to the liposomes, which only reached the upper dermis. The ethosomal formulation of C. heyneana extract exhibited a better anti-ageing activity based on the parameters of epidermal thickness, sunburn cell count, fibroblast count, and the distance between collagen fibres in rat skin histology.
CONCLUSIONS: Ethosomes have been found to be a more proficient carrier system for transdermal delivery of C. heyneana extract compared to liposomes. Meanwhile, their penetration correlated with the effectivity of the formulation, suggesting that the vesicular system enhanced the penetration ability of the extract.
摘要：
目的：本研究旨在开发以姜黄提取物为生物活性物质的抗衰老纳米制剂。
背景：姜黄和Zipj提取物已在先前的研究中被证明具有抗氧化剂，抗衰老,抗炎,和伤口愈合特性，这使得它成为抗衰老和防晒化妆品的潜在生物活性材料。植物抗氧化剂需要渗透到更深的皮肤层以确保有效性。因此，需要透皮递送系统来将提取物递送至更深的皮肤层。
目的：本研究的目的是比较喜鱼根茎乙醇提取物的脂质体和乙醇制剂的通透性和抗衰老活性。
方法：在本研究中，使用乙醇注射方法将Heyneana提取物以脂质体和乙醇体制剂的形式加载到磷脂囊泡系统中。通过分析表皮厚度评估抗衰老活性，晒伤细胞的数量，胶原纤维之间的距离，和成纤维细胞的数量。同时进行组织学标本评分以进行体内渗透研究。
结果：已发现该脂质体制剂具有更好的渗透能力，因为与脂质体相比，它能够到达较低的真皮区域，只到达真皮上部。基于表皮厚度的参数，喜树提取物的组晶型制剂表现出更好的抗衰老活性，晒伤细胞计数,成纤维细胞计数,和大鼠皮肤组织学中胶原纤维之间的距离。
结论：已发现，与脂质体相比，乙醇体是一种更有效的经皮传递喜树提取物的载体系统。同时,它们的渗透与配方的有效性相关，表明囊泡系统增强了提取物的渗透能力。
背景：姜黄和Zipj提取物已在先前的研究中被证明具有抗氧化剂，抗衰老,抗炎,和伤口愈合特性，这使得它成为抗衰老和防晒化妆品的潜在生物活性材料。植物抗氧化剂需要渗透到更深的皮肤层以确保有效性。因此，需要透皮递送系统来将提取物递送至更深的皮肤层。
目的：本研究的目的是比较喜鱼根茎乙醇提取物的脂质体和乙醇制剂的通透性和抗衰老活性。
方法：在本研究中，使用乙醇注射方法将Heyneana提取物以脂质体和乙醇体制剂的形式加载到磷脂囊泡系统中。通过分析表皮厚度评估抗衰老活性，晒伤细胞的数量，胶原纤维之间的距离，和成纤维细胞的数量。同时进行组织学标本评分以进行体内渗透研究。
结果：已发现该脂质体制剂具有更好的渗透能力，因为与脂质体相比，它能够到达较低的真皮区域，只到达真皮上部。基于表皮厚度的参数，喜树提取物的组晶型制剂表现出更好的抗衰老活性，晒伤细胞计数,成纤维细胞计数,和大鼠皮肤组织学中胶原纤维之间的距离。
结论：已发现，与脂质体相比，乙醇体是一种更有效的经皮传递喜树提取物的载体系统。同时,它们的渗透与配方的有效性相关，表明囊泡系统增强了提取物的渗透能力。
