Abstract：
OBJECTIVE: To investigate the possible role of La ribonucleoprotein 7 (LARP7) in psoriasis through a mouse model and uncover its underlying mechanism.
METHODS: The back skin of C57BL/6 mice was smeared with IMquimod (IMQ) cream for 7 days to induce psoriasis. Immunoblot kit was used to detect the deacetylase activity of SIRT1 (member of sirtuin family). Hematoxylin and eosin staining was used to assess the degree of psoriasis in mouse. Flow cytometry assays were performed to confirm effects on Th1/Th17 cell differentiation. Enzyme-linked-immunosorbent serologic assays were used to detect the level of secreted cytokines.
RESULTS: LARP7 upregulated SIRT1 deacetylase activity. LARP7 alleviated psoriasis symptoms in mice by upregulating SIRT1 deacetylase activity. In addition, LARP7 regulated Th1/Th17 cell differentiation in psoriatic mice. We further found that LARP7 inhibited Th1/Th17 cytokine.
CONCLUSIONS: LARP7 upregulated SIRT1 activity and inhibited Th1/Th17 cytokine response in psoriatic mice.
METHODS: The back skin of C57BL/6 mice was smeared with IMquimod (IMQ) cream for 7 days to induce psoriasis. Immunoblot kit was used to detect the deacetylase activity of SIRT1 (member of sirtuin family). Hematoxylin and eosin staining was used to assess the degree of psoriasis in mouse. Flow cytometry assays were performed to confirm effects on Th1/Th17 cell differentiation. Enzyme-linked-immunosorbent serologic assays were used to detect the level of secreted cytokines.
RESULTS: LARP7 upregulated SIRT1 deacetylase activity. LARP7 alleviated psoriasis symptoms in mice by upregulating SIRT1 deacetylase activity. In addition, LARP7 regulated Th1/Th17 cell differentiation in psoriatic mice. We further found that LARP7 inhibited Th1/Th17 cytokine.
CONCLUSIONS: LARP7 upregulated SIRT1 activity and inhibited Th1/Th17 cytokine response in psoriatic mice.
摘要：
目的:通过小鼠模型研究La核糖核蛋白7(LARP7)在银屑病发病中的可能作用,并揭示其作用机制。
方法：用IMQ乳膏涂抹C57BL/6小鼠背部皮肤7天，诱发银屑病。免疫印迹试剂盒用于检测SIRT1（sirtuin家族成员）的脱乙酰酶活性。苏木精和伊红染色用于评估小鼠银屑病的程度。进行流式细胞术测定以确认对Th1/Th17细胞分化的影响。酶联免疫吸附血清学测定用于检测分泌的细胞因子水平。
结果：LARP7上调SIRT1脱乙酰酶活性。LARP7通过上调SIRT1脱乙酰酶活性减轻小鼠银屑病症状。此外,LARP7调控银屑病小鼠Th1/Th17细胞分化。我们进一步发现LARP7抑制Th1/Th17细胞因子。
结论：LARP7上调银屑病小鼠SIRT1活性并抑制Th1/Th17细胞因子反应。
方法：用IMQ乳膏涂抹C57BL/6小鼠背部皮肤7天，诱发银屑病。免疫印迹试剂盒用于检测SIRT1（sirtuin家族成员）的脱乙酰酶活性。苏木精和伊红染色用于评估小鼠银屑病的程度。进行流式细胞术测定以确认对Th1/Th17细胞分化的影响。酶联免疫吸附血清学测定用于检测分泌的细胞因子水平。
结果：LARP7上调SIRT1脱乙酰酶活性。LARP7通过上调SIRT1脱乙酰酶活性减轻小鼠银屑病症状。此外,LARP7调控银屑病小鼠Th1/Th17细胞分化。我们进一步发现LARP7抑制Th1/Th17细胞因子。
结论：LARP7上调银屑病小鼠SIRT1活性并抑制Th1/Th17细胞因子反应。
