PDF(Pubmed)
Abstract：
The mechanism by which arcuate kisspeptin (ARNKISS) neurons co-expressing glutamate, neurokinin B, and dynorphin intermittently synchronize their activity to drive pulsatile hormone secretion remains unclear in females. In order to study spontaneous synchronization within the ARNKISS neuron network, acute brain slices were prepared from adult female Kiss1-GCaMP6 mice. Analysis of both spontaneous synchronizations and those driven by high frequency stimulation of individual ARNKISS neurons revealed that the network exhibits semi-random emergent excitation dependent upon glutamate signaling through AMPA receptors. No role for NMDA receptors was identified. In contrast to male mice, ongoing tachykinin receptor tone within the slice operated to promote spontaneous synchronizations in females. As previously observed in males, we found that ongoing dynorphin transmission in the slice did not contribute to synchronization events. These observations indicate that a very similar AMPA receptor-dependent mechanism underlies ARNKISS neuron synchronizations in the female mouse supporting the \"glutamate two-transition\" model for kisspeptin neuron synchronization. However, a potentially important sex difference appears to exist with a more prominent facilitatory role for tachykinin transmission in the female.
摘要：
弓状kisspeptin（ARNKISS）神经元共表达谷氨酸的机制，神经激肽B,和强啡肽间歇性地同步它们的活动以驱动脉冲激素分泌在女性中仍不清楚。为了研究ARNKISS神经元网络内的自发同步,从成年雌性Kiss1-GCaMP6小鼠制备急性脑切片。对自发同步和由单个ARNKISS神经元的高频刺激驱动的同步的分析显示，该网络表现出半随机紧急兴奋，取决于通过AMPA受体的谷氨酸信号传导。未发现NMDA受体的作用。与雄性小鼠相反，切片中持续的速激肽受体音调可促进女性的自发同步。正如以前在男性中观察到的那样，我们发现切片中持续的强啡肽传输并不促成同步事件.这些观察结果表明，一种非常相似的AMPA受体依赖性机制是雌性小鼠ARNKISS神经元同步的基础，该雌性小鼠支持kisspeptin神经元同步的“谷氨酸双转换”模型。然而,一个潜在的重要性别差异似乎存在，在女性中对速激肽的传播具有更突出的促进作用。
