PDF(Pubmed)
Abstract：
Genes that have been identified in the genome but remain uncharacterized with regards to function offer an opportunity to uncover novel biological information. Novelty is exciting but can also be a barrier. If nothing is known, how does one start planning and executing experiments? Here, we provide a recommended information-mining workflow and a corresponding guide to accessing information about uncharacterized Drosophila melanogaster genes, such as those assigned only a systematic coding gene identifier. The available information can provide insights into where and when the gene is expressed, what the function of the gene might be, whether there are similar genes in other species, whether there are known relationships to other genes, and whether any other features have already been determined. In addition, available information about relevant reagents can inspire and facilitate experimental studies. Altogether, mining available information can help prioritize genes for further study, as well as provide starting points for experimental assays and other analyses.
摘要：
已在基因组中鉴定但在功能方面仍未表征的基因提供了发现新生物学信息的机会。新颖性令人兴奋，但也可能是一个障碍。如果什么都不知道，一个人如何开始计划和执行实验？在这里，我们提供了推荐的信息挖掘工作流程和相应的指南，以访问有关未表征的果蝇基因的信息，例如仅分配了系统编码基因标识符的那些。可用的信息可以提供有关基因表达的地点和时间的见解，基因的功能可能是什么，其他物种是否有相似的基因，是否与其他基因有已知的关系，以及是否已经确定了任何其他特征。此外,有关相关试剂的可用信息可以激发和促进实验研究。总之,挖掘可用信息可以帮助确定基因的优先次序，以便进一步研究，以及提供实验测定和其他分析的起点。
