PDF(Pubmed)
Abstract：
Sleep deprivation (SD) causes several adverse functional outcomes, and understanding the associated processes can improve quality of life. Although the effects of SD on neuronal activity in several brain regions have been identified, a comprehensive evaluation of the whole brain is still lacking. Hence, we performed SD using two different methods, gentle handling and a dedicated chamber, in targeted recombination in active populations 2 (TRAP2) mice crossed with Rosa-ZsGreen reporter mice and visualized cellular activity in the whole brain. Using the semi-automated post-imaging analysis tool Slice Histology Alignment, Registration, and Cell Quantification (SHARCQ), the number of activated cells was quantified. From the analysis of 14 brain regions, cellular activity was significantly increased in the olfactory areas and decreased in the medulla by the two SD methods. From the analysis of the further subdivided 348 regions, cellular activity was significantly increased in the vascular organ of the lamina terminalis, lateral hypothalamic area, parabigeminal nucleus, ventral tegmental area, and magnocellular reticular nucleus, and decreased in the anterior part of the basolateral amygdalar nucleus, nucleus accumbens, septohippocampal nucleus, reticular nucleus of the thalamus, preoptic part of the periventricular hypothalamic nucleus, ventromedial preoptic nucleus, rostral linear nucleus raphe, facial motor nucleus, vestibular nuclei, and some fiber tracts (oculomotor nerve, genu of corpus callosum, and rubrospinal tract) by the two SD methods. Two subdivided regions of the striatum (caudoputamen and other striatum), epithalamus, vascular organ of the lamina terminalis, anteroventral preoptic nucleus, superior colliculus optic layer, medial terminal nucleus of the accessory optic tract, pontine gray, and fiber tracts (medial lemniscus, columns of the fornix, brachium of the inferior colliculus, and mammillary peduncle) were differentially affected by the two SD methods. Most brain regions detected from these analyses have been reported to be involved in regulating sleep/wake regulatory circuits. Moreover, the results from the connectivity analysis indicated that the connectivity of cellular activity among brain regions was altered by SD. Together, such a comprehensive analysis of the whole brain is useful for understanding the mechanisms by which SD and/or sleep disruption affects brain function.
摘要：
睡眠剥夺（SD）导致几种不良的功能结果，了解相关过程可以改善生活质量。尽管已经确定了SD对几个大脑区域神经元活动的影响，对整个大脑的全面评估仍然缺乏。因此，我们使用两种不同的方法进行SD，温和的处理和专用的房间，在活性群体2（TRAP2）小鼠中的靶向重组与Rosa-ZsGreen报告小鼠杂交，并可视化整个大脑中的细胞活性。使用半自动成像后分析工具切片组织学对齐，注册,和细胞定量（SHARCQ），对活化细胞的数量进行定量.通过对14个大脑区域的分析,通过两种SD方法，嗅觉区域的细胞活性显着增加，延髓中的细胞活性降低。从进一步细分的348个地区的分析来看，细胞活性在终末层的血管器官中显著增加，下丘脑外侧区，旁核，腹侧被盖区,和大细胞网状核，并在基底外侧杏仁核的前部减少，伏隔核,海马间核，丘脑网状核,脑室周围下丘脑核的视前部分，腹内侧视前核,延髓线中缝核,面部运动核,前庭核,和一些纤维束（动眼神经，胼胝体甲,和rubrospinal束）通过两种SD方法。纹状体的两个细分区域（尾端和其他纹状体），上丘脑,终末层的血管器官，前腹视前核,上丘视神经层，副视神经束内侧末端核，脑桥灰,和纤维束(内侧板面，穹窿的柱子，下丘的肱,和乳头花梗）受两种SD方法的影响不同。据报道，从这些分析中检测到的大多数大脑区域都参与调节睡眠/觉醒调节回路。此外，连通性分析的结果表明，SD改变了脑区之间细胞活动的连通性。一起,这种对整个大脑的全面分析对于理解SD和/或睡眠中断影响大脑功能的机制很有用。
