PDF(Pubmed)
Abstract：
As an important food and cash crop, identification of DNA molecular markers is of great significance for molecular marker-assisted breeding of Sorghum (Sorghum bicolor (L.) moench). Although some sorghum-related mutation databases have been published, the special SSR and SV databases still need to be constructed and updated.
In this study, the quality of 18 different sorghum genomes was evaluated, and two genomes were assembled at chromosome level. Through the identification and comparative analysis of SSR loci in these genomes, the distribution characteristics of SSR in the above sorghum genomes were initially revealed. At the same time, five representative reference genomes were selected to identify the structural variation of sorghum. Finally, a convenient SSR/SV database of sorghum was constructed by integrating the above results ( http://www.sorghum.top:8079/ ; http://43.154.129.150:8079/ ; http://47.106.184.91:8079/ ). Users can query the information of related sites and primer pairs.
Anyway, our research provides convenience for sorghum researchers and will play an active role in sorghum molecular marker-assisted breeding.
In this study, the quality of 18 different sorghum genomes was evaluated, and two genomes were assembled at chromosome level. Through the identification and comparative analysis of SSR loci in these genomes, the distribution characteristics of SSR in the above sorghum genomes were initially revealed. At the same time, five representative reference genomes were selected to identify the structural variation of sorghum. Finally, a convenient SSR/SV database of sorghum was constructed by integrating the above results ( http://www.sorghum.top:8079/ ; http://43.154.129.150:8079/ ; http://47.106.184.91:8079/ ). Users can query the information of related sites and primer pairs.
Anyway, our research provides convenience for sorghum researchers and will play an active role in sorghum molecular marker-assisted breeding.
摘要：
背景：作为重要的粮食作物和经济作物，DNA分子标记的鉴定对于高粱的分子标记辅助育种具有重要意义（高粱双色（L.）莫恩奇）。尽管一些高粱相关的突变数据库已经发表，特殊的SSR和SV数据库仍然需要构建和更新。
结果：在这项研究中，对18个不同的高粱基因组的质量进行了评估，在染色体水平上组装了两个基因组。通过对这些基因组中SSR位点的鉴定和比较分析,初步揭示了SSR在上述高粱基因组中的分布特征。同时,选择了五个具有代表性的参考基因组来鉴定高粱的结构变异。最后,通过整合上述结果，构建了一个方便的高粱SSR/SV数据库（http：//www。高粱.顶部:8079/;http://43.154.129.150:8079/;http://47.106.184.91:8079/)。用户可以查询相关站点和引物对的信息。
结论：无论如何，本研究为高粱研究人员提供了便利，并将在高粱分子标记辅助育种中发挥积极作用。
结果：在这项研究中，对18个不同的高粱基因组的质量进行了评估，在染色体水平上组装了两个基因组。通过对这些基因组中SSR位点的鉴定和比较分析,初步揭示了SSR在上述高粱基因组中的分布特征。同时,选择了五个具有代表性的参考基因组来鉴定高粱的结构变异。最后,通过整合上述结果，构建了一个方便的高粱SSR/SV数据库（http：//www。高粱.顶部:8079/;http://43.154.129.150:8079/;http://47.106.184.91:8079/)。用户可以查询相关站点和引物对的信息。
结论：无论如何，本研究为高粱研究人员提供了便利，并将在高粱分子标记辅助育种中发挥积极作用。
