Abstract：
Orotic aciduria is a severe, hereditary, life-threatening condition, particularly in newborns. An increased orotic acid (OA) content in urine may be a strong indicator of this condition. In this study, we developed a rapid, simple, highly sensitive diagnostic method for use in monitoring the OA levels in urine samples, which were successfully determined using capillary electrophoresis combined with capacitively coupled contactless conductivity detection (CE-C4D). A straightforward analysis with an increased sensitivity towards OA and an analysis time of approximately 5 min were realized, and the limit of detection of the developed method was 0.014 mg/L in aqueous solution. The optimized composition of the separation electrolyte was 20 mM 2-(N-morpholino)ethanesulfonic acid/histidine with 0.1 mM cetyltrimethylammonium bromide at a pH of 6.5. The sensitivity of the developed method was significantly increased using a sample stacking approach with a 10% acetonitrile (v/v) plug solution. The method was validated, and satisfactory recoveries of 80.0-92.3% were obtained. The amounts of OA in five urine samples were successfully determined.
摘要：
嗜酸尿症是一种严重的，世袭,危及生命的状况,尤其是新生儿。尿液中乳清酸（OA）含量的增加可能是这种情况的有力指标。在这项研究中,我们开发了一种快速的，简单,用于监测尿样中OA水平的高灵敏度诊断方法，使用毛细管电泳结合电容耦合非接触电导检测（CE-C4D）成功确定。实现了对OA灵敏度增加的简单分析，分析时间约为5分钟，该方法在水溶液中的检出限为0.014mg/L。分离电解质的优化组成是20mM2-(N-吗啉代)乙磺酸/组氨酸和0.1mM十六烷基三甲基溴化铵,pH为6.5。使用具有10%乙腈(v/v)塞溶液的样品堆叠方法,所开发的方法的灵敏度显著提高。对该方法进行了验证,回收率为80.0-92.3%。成功测定了5份尿样中OA的含量。
