Abstract：
OBJECTIVE: Granulocyte transfusion (GTX) is a treatment option for severe infections in patients with neutropenia. In previous studies, hydroxyethyl starch (HES) was used to enhance red blood cell sedimentation for granulocyte collection (GC). However, there are safety concerns about HES, and HES is not readily available in some countries. Therefore, we compared the granulocyte counts and GC efficiency achieved by two apheresis systems without HES.
METHODS: All consecutive GC procedures performed between July 2011 and March 2018 at our hospital were analysed. COBE Spectra was used until 5 February 2016, and Spectra Optia was used afterwards. HES was not used.
RESULTS: Twenty-six GC procedures were performed, including 18 performed using COBE Spectra and 8 using Spectra Optia. When Spectra Optia was used, >1 × 1010 neutrophils were collected from seven of the eight (88%) procedures. Although there was no significant difference in the granulocyte yield between COBE Spectra-based and Spectra Optia-based GC procedures, the collection efficiency of Spectra Optia was significantly higher than that of COBE Spectra (p = 0.021). Furthermore, the granulocyte yields of Spectra Optia-based GC tended to be more strongly correlated with the peripheral blood neutrophil count on the day of apheresis than those of COBE Spectra-based GC.
CONCLUSIONS: Our results suggest that Spectra Optia achieves greater GC efficiency than COBE Spectra, even without HES. GTX may be a therapeutic option for severe neutropenia, even in places where HES is not available.
METHODS: All consecutive GC procedures performed between July 2011 and March 2018 at our hospital were analysed. COBE Spectra was used until 5 February 2016, and Spectra Optia was used afterwards. HES was not used.
RESULTS: Twenty-six GC procedures were performed, including 18 performed using COBE Spectra and 8 using Spectra Optia. When Spectra Optia was used, >1 × 1010 neutrophils were collected from seven of the eight (88%) procedures. Although there was no significant difference in the granulocyte yield between COBE Spectra-based and Spectra Optia-based GC procedures, the collection efficiency of Spectra Optia was significantly higher than that of COBE Spectra (p = 0.021). Furthermore, the granulocyte yields of Spectra Optia-based GC tended to be more strongly correlated with the peripheral blood neutrophil count on the day of apheresis than those of COBE Spectra-based GC.
CONCLUSIONS: Our results suggest that Spectra Optia achieves greater GC efficiency than COBE Spectra, even without HES. GTX may be a therapeutic option for severe neutropenia, even in places where HES is not available.
摘要：
目的：粒细胞输注（GTX）是中性粒细胞减少症患者严重感染的一种治疗选择。在以往的研究中,羟乙基淀粉（HES）用于增强红细胞沉降以收集粒细胞（GC）。然而,有关于HES的安全问题，和HES在某些国家并不容易获得。因此,我们比较了两种无HES的单采系统的粒细胞计数和GC效率。
方法：分析2011年7月至2018年3月在我院进行的所有连续GC手术。使用COBE光谱直到2016年2月5日，之后使用光谱。未使用HES。
结果：进行了26个GC程序，包括使用COBE光谱进行的18和使用光谱光学进行的8。当使用SpectraOptia时，从八个程序中的七个（88％）收集>1×1010个嗜中性粒细胞。尽管基于COBE光谱和基于光谱的GC程序之间的粒细胞产量没有显着差异，光谱的收集效率明显高于COBE光谱(p=0.021)。此外，与基于COBE光谱的GC相比，基于光谱的GC在单采当天的粒细胞产量与外周血中性粒细胞计数的相关性更强。
结论：我们的结果表明，光谱光学比COBE光谱具有更大的GC效率，即使没有他。GTX可能是严重中性粒细胞减少症的治疗选择，即使在HES不可用的地方。
方法：分析2011年7月至2018年3月在我院进行的所有连续GC手术。使用COBE光谱直到2016年2月5日，之后使用光谱。未使用HES。
结果：进行了26个GC程序，包括使用COBE光谱进行的18和使用光谱光学进行的8。当使用SpectraOptia时，从八个程序中的七个（88％）收集>1×1010个嗜中性粒细胞。尽管基于COBE光谱和基于光谱的GC程序之间的粒细胞产量没有显着差异，光谱的收集效率明显高于COBE光谱(p=0.021)。此外，与基于COBE光谱的GC相比，基于光谱的GC在单采当天的粒细胞产量与外周血中性粒细胞计数的相关性更强。
结论：我们的结果表明，光谱光学比COBE光谱具有更大的GC效率，即使没有他。GTX可能是严重中性粒细胞减少症的治疗选择，即使在HES不可用的地方。
