Abstract：
The reproductive toxicity of polycyclic aromatic hydrocarbons (PAHs) in aquatic organisms has attracted increasing attention from scholars. Currently, research in this field primarily focuses on vertebrates such as zebrafish and other model species. However, there is still a significant knowledge gap in the toxicity of PAHs to invertebrates and its potential mechanisms. Benzo[a]pyrene (B[a]P) is one of the most representative PAHs. In this study, female Ruditapes philippinarum (R. philippinarum) was treated with B[a]P concentrations of 0, 0.8, 4, and 20 μg/L to investigate reproductive indicators in the proliferative, growth, mature, and spawn stages. Transcriptomics was used to investigate the expression of genes associated with the reproductive endocrine system, DNA repair, autophagy, apoptosis, and ovarian development at different reproductive stages. Our results suggested that B[a]P disrupted the endocrine system by interfering with the production of steroid hormones and the transmission of estrogen signals in female R. philippinarum. The structure of the ovarian DNA duplex is severely damaged under the stress of B[a]P, and a series of cellular responses caused by DNA damage are also interfered. Additionally, we observed a reduction in the gonadosomatic index (GSI) and mature oocytes numbers after B[a]P exposed. Tissue section indicated that severe damage to the ovarian structure at mature and spawn stages. In conclusion, this study combined transcriptomic and toxicological to explore the negative effects on ovarian development induced by B[a]P, focusing on reproductive endocrine disturbance and DNA damage.
摘要：
多环芳烃(PAHs)对水生生物的生殖毒性越来越受到学者们的关注。目前,该领域的研究主要集中在斑马鱼和其他模型物种等脊椎动物上。然而,在PAHs对无脊椎动物的毒性及其潜在机制方面仍然存在很大的知识差距。苯并[a]芘(B[a]P)是最具代表性的PAHs之一。在这项研究中,菲律宾女鹿(R.菲律宾）用浓度为0、0.8、4和20μg/L的B[a]P处理，以研究增殖中的生殖指标，增长,成熟,和产卵阶段。转录组学用于研究与生殖内分泌系统相关的基因的表达，DNA修复,自噬,凋亡，和卵巢发育在不同的生殖阶段。我们的结果表明，B[a]P通过干扰雌性菲律宾R.的类固醇激素的产生和雌激素信号的传递来破坏内分泌系统。卵巢DNA双链体的结构在B[a]P的胁迫下严重受损，DNA损伤引起的一系列细胞反应也受到干扰。此外,我们观察到B[a]P暴露后，性腺指数（GSI）和成熟卵母细胞数量减少。组织切片表明卵巢结构在成熟和产卵阶段严重受损。总之,本研究结合转录组学和毒理学，探讨B[a]P对卵巢发育的负面影响，重点研究生殖内分泌紊乱和DNA损伤。
