Abstract：
In this work the cosmetic preservative based on a Ribes Nigrum (blackcurrant) plant extract (PhytoCide Black Currant Powder abbr. BCE) was investigated to evaluate its antibacterial effect and to gain an insight into its mechanism of action. The influence of this commercially available formulation on model Escherichia coli and Staphylococcus aureus lipid membranes was studied to analyze its interactions with membrane lipids at a molecular level. The mixed lipid monolayers and one component bacteria lipid films were used to investigate the effect of BCE on condensation and morphology of model systems and to study the ability of BCE components to penetrate into the lipid environment. The in vitro tests were also done on different bacteria species (E. coli, Enterococcus faecalis, S. aureus, Salmonella enterica, Pseudomonas aeruginosa) to compare antimicrobial potency of the studied formulation. As evidenced the in vitro studies BCE formulation exerts very similar antibacterial activity against E. coli and S. aureus. Moreover, based on the collected data it is impossible to indicate which bacteria: Gram-positive or Gram-negative are more susceptible to this formulation. Model membrane experiments evidenced that the studied preservative affects organization of both E. coli and S. aureus model system by decreasing their condensation and altering their morphology. BCE components are able to penetrate into the lipid systems. However, all these effects depend on the lipid composition and monolayer organization. The collected results were analyzed from the point of view of the mechanism of action of blackcurrant extract and the factors, which may determine the activity of this formulation.
摘要：
在这项工作中，基于RibesNigrum（黑醋栗）植物提取物（PhytoCide黑醋栗粉末abbr。对BCE）进行了研究，以评估其抗菌作用并深入了解其作用机理。研究了该市售制剂对模型大肠杆菌和金黄色葡萄球菌脂质膜的影响，以在分子水平上分析其与膜脂质的相互作用。使用混合脂质单层和单组分细菌脂质膜来研究BCE对模型体系的缩合和形态的影响，并研究BCE组分渗透到脂质环境中的能力。还对不同的细菌种类进行了体外测试(E.大肠杆菌粪肠球菌,金黄色葡萄球菌，肠沙门氏菌,铜绿假单胞菌）比较所研究制剂的抗微生物效力。如体外研究证明的,BCE制剂对大肠杆菌和金黄色葡萄球菌具有非常相似的抗菌活性。此外，根据收集的数据，不可能指出革兰氏阳性或革兰氏阴性细菌对这种制剂更敏感。模型膜实验证明,所研究的防腐剂通过减少它们的缩合和改变它们的形态来影响大肠杆菌和金黄色葡萄球菌模型系统的组织。BCE组分能够渗透到脂质系统中。然而,所有这些影响取决于脂质组成和单层组织。从黑加仑提取物的作用机理和影响因素的角度分析了收集的结果，这可以确定该制剂的活性。
