Abstract：
UNASSIGNED: To investigate whether the Anti-Müllerian Hormone (AMH), an ovarian hormone belonging to the Transforming Growth Factor β superfamily, may represent a possible candidate for use as a bone anabolic factor.
UNASSIGNED: We performed in vitro studies on Human Osteoblasts (HOb) to evaluate the expression and the functionality of AMHRII, the AMH receptor type-2, and investigate the effects of exogenous AMH exposure on osteogenic gene expression and osteoblast functions.
UNASSIGNED: We reported the first evidence for the expression and functionality of AMHRII in HOb cells, thus suggesting that osteoblasts may represent a specific target for exogenous AMH treatment. Furthermore, the exposure to AMH exerted a stimulatory effect on HOb cells leading to the activation of osteogenic genes, including the upregulation of osteoblastic transcription factors such as RUNX and OSX, along with increased deposition of mineralized nodules.
UNASSIGNED: Our findings proved interesting clues on the stimulatory effects of AMH on mature osteoblasts expressing its specific receptor, AMHRII. This study may therefore have translation value in opening the perspective that AMH may be an effective candidate to counteract the bone loss in osteoporotic patients by selectively targeting osteoblast with minimal off-target effect.
UNASSIGNED: We performed in vitro studies on Human Osteoblasts (HOb) to evaluate the expression and the functionality of AMHRII, the AMH receptor type-2, and investigate the effects of exogenous AMH exposure on osteogenic gene expression and osteoblast functions.
UNASSIGNED: We reported the first evidence for the expression and functionality of AMHRII in HOb cells, thus suggesting that osteoblasts may represent a specific target for exogenous AMH treatment. Furthermore, the exposure to AMH exerted a stimulatory effect on HOb cells leading to the activation of osteogenic genes, including the upregulation of osteoblastic transcription factors such as RUNX and OSX, along with increased deposition of mineralized nodules.
UNASSIGNED: Our findings proved interesting clues on the stimulatory effects of AMH on mature osteoblasts expressing its specific receptor, AMHRII. This study may therefore have translation value in opening the perspective that AMH may be an effective candidate to counteract the bone loss in osteoporotic patients by selectively targeting osteoblast with minimal off-target effect.
摘要：
■为了调查抗苗勒管激素(AMH)属于转化生长因子β超家族的卵巢激素，可能代表用作骨合成代谢因子的可能候选者。
■我们对人类成骨细胞（HOb）进行了体外研究，以评估AMHRII的表达和功能，AMH受体2型，探讨外源性AMH暴露对成骨基因表达和成骨细胞功能的影响。
■我们报道了AMHRII在HOb细胞中表达和功能的第一个证据，因此表明成骨细胞可能是外源性AMH治疗的特异性靶标。此外，暴露于AMH对HOb细胞产生刺激作用，导致成骨基因的激活，包括RUNX和OSX等成骨细胞转录因子的上调，随着矿化结核沉积的增加。
■我们的发现证明了AMH对表达其特异性受体的成熟成骨细胞的刺激作用的有趣线索，AMHRII。因此，这项研究可能具有翻译价值，可以打开以下观点：AMH可能是通过选择性靶向成骨细胞而以最小的脱靶效应来抵消骨质疏松患者骨丢失的有效候选者。
■我们对人类成骨细胞（HOb）进行了体外研究，以评估AMHRII的表达和功能，AMH受体2型，探讨外源性AMH暴露对成骨基因表达和成骨细胞功能的影响。
■我们报道了AMHRII在HOb细胞中表达和功能的第一个证据，因此表明成骨细胞可能是外源性AMH治疗的特异性靶标。此外，暴露于AMH对HOb细胞产生刺激作用，导致成骨基因的激活，包括RUNX和OSX等成骨细胞转录因子的上调，随着矿化结核沉积的增加。
■我们的发现证明了AMH对表达其特异性受体的成熟成骨细胞的刺激作用的有趣线索，AMHRII。因此，这项研究可能具有翻译价值，可以打开以下观点：AMH可能是通过选择性靶向成骨细胞而以最小的脱靶效应来抵消骨质疏松患者骨丢失的有效候选者。
