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Abstract：
BACKGROUND: Highly upregulated in liver cancer (HULC) is one of the LncRNAs that was documented to enhance cancer progression, and its downregulation is associated with cell cycle arrest and apoptosis. Myotubularin-related protein 3 (MTMR3) is required for autophagy, and many studies consider MTMR3 to be a negative regulator of autophagy processes. However, nothing is understood about how they regulate breast cancer.
METHODS: This case-control study included 245 patients (Group A: 85 early BC Group B: 40 metastatic BC cases, Group C: 40 fibroadenoma cases; and Group D: 80 age matched healthy control subjects. TaqMan Real-time PCR was used to analyse rs7158663 and rs12537. MTMR3 and HULC gene expression levels were measured using RT-PCR.
RESULTS: Breast cancer patients exhibited elevated serum MTMR3 and HULC compared to fibroadenomas and control cases. The MTMR3 rs12537 \"T/T\" genotype was highly expressed in cases of breast cancer (early and metastatic) compared to controls (risk genotype). On the other hand, the HULC rs7158663 genotypes were not statistically associated with breast cancer. However, when compared to the control, the C/C genotype of the HULC gene is higher in the case.MTMR3 gene expression was higher in the T/T genotype compared to both the C/C and C/T genotypes, while HULC gene expression was lower in the A/C genotype compared to both the A/A and C/C genotypes. Positive correlation between MTMR3 and HULC. MTMR3 and ALT, as well as HULC and alkaline phosphatase, both showed a statistically significant positive correlation.
CONCLUSIONS: Our findings reveal that MTMR3 and HULC serum expression and their SNPs (HULC rs7763881, MTMR3 rs12537) are associated with a higher risk for the development of breast cancer in the Egyptian population.
METHODS: This case-control study included 245 patients (Group A: 85 early BC Group B: 40 metastatic BC cases, Group C: 40 fibroadenoma cases; and Group D: 80 age matched healthy control subjects. TaqMan Real-time PCR was used to analyse rs7158663 and rs12537. MTMR3 and HULC gene expression levels were measured using RT-PCR.
RESULTS: Breast cancer patients exhibited elevated serum MTMR3 and HULC compared to fibroadenomas and control cases. The MTMR3 rs12537 \"T/T\" genotype was highly expressed in cases of breast cancer (early and metastatic) compared to controls (risk genotype). On the other hand, the HULC rs7158663 genotypes were not statistically associated with breast cancer. However, when compared to the control, the C/C genotype of the HULC gene is higher in the case.MTMR3 gene expression was higher in the T/T genotype compared to both the C/C and C/T genotypes, while HULC gene expression was lower in the A/C genotype compared to both the A/A and C/C genotypes. Positive correlation between MTMR3 and HULC. MTMR3 and ALT, as well as HULC and alkaline phosphatase, both showed a statistically significant positive correlation.
CONCLUSIONS: Our findings reveal that MTMR3 and HULC serum expression and their SNPs (HULC rs7763881, MTMR3 rs12537) are associated with a higher risk for the development of breast cancer in the Egyptian population.
摘要：
背景：在肝癌（HULC）中高度上调是LncRNAs之一，被证明可以促进癌症进展，其下调与细胞周期停滞和细胞凋亡有关。肌管蛋白相关蛋白3（MTMR3）是自噬所必需的，许多研究认为MTMR3是自噬过程的负调节因子.然而,对它们如何调节乳腺癌一无所知。
方法：本病例对照研究共纳入245例患者（A组：早期BC85例B组：转移性BC40例，C组：40例纤维腺瘤；D组：80例年龄匹配的健康对照组。使用TaqMan实时PCR分析rs7158663和rs12537。使用RT-PCR测量MTMR3和HULC基因表达水平。
结果：与纤维腺瘤和对照组相比，乳腺癌患者的血清MTMR3和HULC升高。与对照组（风险基因型）相比，MTMR3rs12537“T/T”基因型在乳腺癌（早期和转移性）病例中高表达。另一方面,HULCrs7158663基因型与乳腺癌无统计学关联.然而,与对照相比，HULC基因的C/C基因型较高。与C/C和C/T基因型相比，T/T基因型的MTMR3基因表达更高，与A/A和C/C基因型相比，A/C基因型的HULC基因表达较低。MTMR3与HULC呈正相关。MTMR3和ALT，以及HULC和碱性磷酸酶，两者呈统计学显著正相关。
结论：我们的研究结果表明，在埃及人群中，MTMR3和HULC血清表达及其SNP（HULCrs7763881，MTMR3rs12537）与更高的乳腺癌发展风险相关。
方法：本病例对照研究共纳入245例患者（A组：早期BC85例B组：转移性BC40例，C组：40例纤维腺瘤；D组：80例年龄匹配的健康对照组。使用TaqMan实时PCR分析rs7158663和rs12537。使用RT-PCR测量MTMR3和HULC基因表达水平。
结果：与纤维腺瘤和对照组相比，乳腺癌患者的血清MTMR3和HULC升高。与对照组（风险基因型）相比，MTMR3rs12537“T/T”基因型在乳腺癌（早期和转移性）病例中高表达。另一方面,HULCrs7158663基因型与乳腺癌无统计学关联.然而,与对照相比，HULC基因的C/C基因型较高。与C/C和C/T基因型相比，T/T基因型的MTMR3基因表达更高，与A/A和C/C基因型相比，A/C基因型的HULC基因表达较低。MTMR3与HULC呈正相关。MTMR3和ALT，以及HULC和碱性磷酸酶，两者呈统计学显著正相关。
结论：我们的研究结果表明，在埃及人群中，MTMR3和HULC血清表达及其SNP（HULCrs7763881，MTMR3rs12537）与更高的乳腺癌发展风险相关。
