Abstract：
Endometriotic cells exhibit a notable degree of invasiveness and some characteristics of tissue remodeling underlying lesion formation. In this regard, do matrix metalloproteinases 14 (MMP14) and other related genes such as SPARC-like protein 1 (SPARCL1), caveolin 2 (CAV2), and clusterin (CLU) exert any significant influence in the processes of endometriosis development and pathophysiology is not apparent. We aim to assess whether these genes could serve as potential diagnostic biomarkers in endometriosis. Microarray-based gene expression analysis was performed on total RNA extracted from endometriotic tissue samples treated with and without gonadotropin-releasing hormone agonist (GnRHa). The GnRHa untreated patients were considered the control group. The validation of genes was performed using quantitative real-time polymerase chain reaction (qRT-PCR). qRT-PCR analysis showed significant downregulation in the expression of MMP14 (p = 0.024), CAV2 (p = 0.017), and upregulation of CLU (p = 0.005) in endometriosis patients treated with GnRHa. SPARCL1 did not show any significant (p = 0.30) change in the expression compared to the control group. These data have the potential to contribute to the comprehension of the molecular pathways implicated in the remodeling of the extracellular matrix, which is a vital step for the physiology of the endometrium. Based on the result, it is concluded that changes in the expression of MMP14, CAV2, and CLU post-treatment imply their role in the pathophysiology of endometriosis and may serve as a potential diagnostic biomarker of endometriosis in response to GnRHa treatment in patients with ovarian endometrioma.
摘要：
子宫内膜异位症细胞表现出明显的侵袭性和一些组织重塑的特征，这些特征是病变形成的基础。在这方面,基质金属蛋白酶14（MMP14）和其他相关基因，如SPARC样蛋白1（SPARCL1），小窝蛋白2（CAV2），而clusterin(CLU)在子宫内膜异位症的发展和病理生理过程中发挥着任何显著的影响。我们旨在评估这些基因是否可以作为子宫内膜异位症的潜在诊断生物标志物。对从用和不用促性腺激素释放激素激动剂(GnRHa)处理的子宫内膜异位组织样品中提取的总RNA进行基于微阵列的基因表达分析。未治疗的GnRHa患者被认为是对照组。使用定量实时聚合酶链反应(qRT-PCR)进行基因的验证。qRT-PCR分析显示MMP14表达显著下调(p=0.024),CAV2（p=0.017），GnRHa治疗的子宫内膜异位症患者CLU上调(p=0.005)。与对照组相比,SPARCL1的表达没有显示出任何显著的变化(p=0.30)。这些数据有可能有助于理解与细胞外基质重塑有关的分子途径，这是子宫内膜生理学的重要一步。根据结果,结论MMP14、CAV2和CLU治疗后表达的变化暗示了它们在子宫内膜异位症的病理生理学中的作用,并可能作为卵巢子宫内膜异位症患者GnRHa治疗的潜在诊断性生物标志物。
