PDF(Pubmed)
Abstract：
Tissue engineering and cell therapy for regenerative medicine have great potential to treat chronic disorders. In musculoskeletal disorders, mesenchymal stromal cells (MSCs) have been identified as a relevant cell type in cell and regenerative strategies due to their multi-lineage potential, although this is likely to be a result of their trophic and immunomodulatory effects on other cells. This PRISMA systematic review aims to assess whether the age of the patient influences the chondrogenic potential of MSCs in regenerative therapy. We identified a total of 3027 studies after performing a search of four databases, including Cochrane, Web of Science, Medline, and PubMed. After applying inclusion and exclusion criteria, a total of 14 papers were identified that were reviewed, assessed, and reported. Cell surface characterization and proliferation, as well as the osteogenic, adipogenic, and chondrogenic differentiation, were investigated as part of the analysis of these studies. Most included studies suggest a clear link between aged donor MSCs and diminished clonogenic and proliferative potential. Our study reveals a heterogeneous and conflicting range of outcomes concerning the chondrogenic, osteogenic, and adipogenic potential of MSCs in relation to age. Further investigations on the in vitro effects of chronological age on the chondrogenic potential of MSCs should follow the outcomes of this systematic review, shedding more light on this complex relationship.
摘要：
用于再生医学的组织工程和细胞疗法在治疗慢性疾病方面具有巨大潜力。在肌肉骨骼疾病中，间充质基质细胞（MSCs）由于其多谱系潜力，已被确定为细胞和再生策略中的相关细胞类型，尽管这可能是它们对其他细胞的营养和免疫调节作用的结果。这项PRISMA系统评价旨在评估患者的年龄是否会影响再生治疗中MSC的软骨形成潜能。在搜索四个数据库后，我们确定了总共3027项研究，包括Cochrane,WebofScience,Medline,和PubMed。在应用纳入和排除标准后，共确定了14篇论文进行了审查，评估,并报告。细胞表面表征和增殖，以及成骨，成脂,和软骨分化，作为这些研究分析的一部分进行了调查。大多数纳入的研究表明，衰老的供体MSC与克隆形成和增殖潜力降低之间存在明确的联系。我们的研究揭示了关于软骨形成的异质性和冲突的结果范围，成骨,和骨髓间充质干细胞成脂潜能与年龄的关系。根据本系统综述的结果，进一步研究实际年龄对MSCs软骨形成潜能的体外影响。对这种复杂的关系有更多的了解。
