PDF(Pubmed)
Abstract：
Species within the genus Equus are valued for their draft ability. Skeletal muscle forms the foundation of the draft ability of Equus species; however, skeletal muscle development-related conserved genes and their target miRNAs are rarely reported for Equus. In this study, a comparative genomics analysis was performed among five species (horse, donkey, zebra, cattle, and goat), and the results showed that a total of 15,262 (47.43%) genes formed the core gene set of the five species. Only nine chromosomes (Chr01, Chr02, Chr03, Chr06, Chr10, Chr18, Chr22, Chr27, Chr29, and Chr30) exhibited a good collinearity relationship among Equus species. The micro-synteny analysis results showed that TPM3 was evolutionarily conserved in chromosome 1 in Equus. Furthermore, donkeys were used as the model species for Equus to investigate the genetic role of TPM3 in muscle development. Interestingly, the results of comparative transcriptomics showed that the TPM3 gene was differentially expressed in donkey skeletal muscle S1 (2 months old) and S2 (24 months old), as verified via RT-PCR. Dual-luciferase test analysis showed that the TPM3 gene was targeted by differentially expressed miRNA (eca-miR-1). Furthermore, a total of 17 TPM3 gene family members were identified in the whole genome of donkey, and a heatmap analysis showed that EaTPM3-5 was a key member of the TPM3 gene family, which is involved in skeletal muscle development. In conclusion, the TPM3 gene was conserved in Equus, and EaTPM3-5 was targeted by eca-miR-1, which is involved in skeletal muscle development in donkeys.
摘要：
马属中的物种因其草稿能力而受到重视。骨骼肌构成了马属物种牵引能力的基础；然而，骨骼肌发育相关的保守基因及其靶miRNAs在马的报道很少。在这项研究中,在五个物种(马，驴,斑马,牛,和山羊），结果表明，共有15,262个基因（47.43％）组成了这五个物种的核心基因集。只有9条染色体（Chr01，Chr02，Chr03，Chr06，Chr10，Chr18，Chr22，Chr27，Chr29和Chr30）在马属物种之间表现出良好的共线性关系。微同系性分析结果表明，TPM3在1号染色体上是进化保守的。此外，驴被用作马属的模型种，以研究TPM3在肌肉发育中的遗传作用。有趣的是,比较转录组学结果表明，TPM3基因在驴骨骼肌S1（2月龄）和S2（24月龄）中差异表达，通过RT-PCR验证。双荧光素酶检测分析表明TPM3基因被差异表达miRNA(eca-miR-1)靶向。此外，在驴全基因组中共鉴定出17个TPM3基因家族成员,热图分析表明EaTPM3-5是TPM3基因家族的关键成员，参与骨骼肌发育。总之,TPM3基因在马科斯是保守的，EaTPM3-5被eca-miR-1靶向，它参与驴骨骼肌发育。
