PDF(Pubmed)
Abstract：
Winter wheat is used as forage at the tillering stage in many countries; however, the regrowth pattern of wheat after mowing remains unclear. In this study, the growth patterns of wheat were revealed through cytological and physiological assessments as well as transcriptome sequencing. The results of agronomic traits and paraffin sections showed that the shoot growth rate increased, but root growth was inhibited after mowing. The submicroscopic structure revealed a decrease in heterochromatin in the tillering node cell and a change in mitochondrial shape in the tillering node and secondary root. Analysis of the transcriptome showed the number of differentially expressed genes (DEGs) involved in biological processes, cellular components, and molecular functions; 2492 upregulated DEGs and 1534 downregulated DEGs were identified. The results of the experimental study showed that mowing induced expression of DEGs in the phenylpropanoid biosynthesis pathway and increased the activity of PAL and 4CL. The upregulated DEGs in the starch and sucrose metabolism pathways and related enzyme activity alterations indicated that the sugar degradation rate increased. The DEGs in the nitrogen metabolism pathway biosynthesis of the amino acids, phenylpropanoid biosynthesis metabolism, and in the TCA pathway also changed after mowing. Hormone content and related gene expression was also altered in the tillering and secondary roots after mowing. When jasmonic acid and ethylene were used to treat the wheat after mowing, the regeneration rate increased, whereas abscisic acid inhibited regrowth. This study revealed the wheat growth patterns after mowing, which could lead to a better understanding of the development of dual-purpose wheat.
摘要：
在许多国家，冬小麦在分耕阶段被用作牧草；然而，小麦割草后的再生长模式尚不清楚。在这项研究中,通过细胞学和生理学评估以及转录组测序揭示了小麦的生长模式。农艺性状和石蜡切片结果表明，枝条生长速率增加，但割草后根系生长受到抑制。亚显微结构显示分till节点细胞中异染色质减少，分till节点和次生根的线粒体形状发生变化。转录组分析表明，参与生物过程的差异表达基因（DEGs）的数量，细胞成分，和分子功能；鉴定出2492个上调的DEGs和1534个下调的DEGs。实验研究结果表明，割草在苯丙素生物合成途径中诱导了DEGs的表达，并增加了PAL和4CL的活性。淀粉和蔗糖代谢途径中DEGs的上调和相关酶活性的改变表明糖降解速率增加。DEGs在氮代谢途径中的氨基酸生物合成,苯丙素生物合成代谢，而TCA途径在割草后也发生了改变。割草后，分till和次生根中的激素含量和相关基因表达也发生了变化。当茉莉酸和乙烯用于处理割草后的小麦时，再生率增加，而脱落酸抑制再生长。这项研究揭示了小麦割草后的生长模式，这可以更好地理解两用小麦的发展。
