PDF(Pubmed)
Abstract：
Among malignant neoplasms, pancreatic ductal adenocarcinoma (PDAC) has one of the highest fatality rates due to its late detection. Therefore, it is essential to discover a noninvasive, early, specific, and sensitive diagnostic method. MicroRNAs (miRNAs) are attractive biomarkers because they are accessible, highly specific, and sensitive. It is crucial to find miRNAs that could be used as possible biomarkers because PDAC is the eighth most common cause of cancer death in Mexico. With the help of microRNA microarrays, differentially expressed miRNAs (DEmiRNAs) were found in PDAC tissues. The presence of these DEmiRNAs in the plasma of Mexican patients with PDAC was determined using RT-qPCR. Receiver operating characteristic curve analysis was performed to determine the diagnostic capacity of these DEmiRNAs. Gene Expression Omnibus datasets (GEO) were employed to verify our results. The Prisma V8 statistical analysis program was used. Four DEmiRNAs in plasma from PDAC patients and microarray tissues were found. Serum samples from patients with PDAC were used to validate their overexpression in GEO databases. We discovered a new panel of the two miRNAs miR-222-3p and miR-221-3p that could be used to diagnose PDAC, and when miR-221-3p and miR-222-3p were overexpressed, survival rates decreased. Therefore, miR-222-3p and miR-221-3p might be employed as noninvasive indicators for the diagnosis and survival of PDAC in Mexican patients.
摘要：
在恶性肿瘤中，胰腺导管腺癌(PDAC)因其晚期发现而成为致死率最高的疾病之一.因此,发现一种非侵入性的，早期，具体,和灵敏的诊断方法。microRNAs（miRNAs）是有吸引力的生物标志物，高度特异性,和敏感。找到可用作生物标志物的miRNA至关重要，因为PDAC是墨西哥癌症死亡的第八大常见原因。在microRNA微阵列的帮助下，在PDAC组织中发现差异表达的miRNA(DEmiRNA)。使用RT-qPCR确定患有PDAC的墨西哥患者的血浆中这些DEmiRNA的存在。进行受试者工作特征曲线分析以确定这些DEmiRNA的诊断能力。使用基因表达综合数据集(GEO)来验证我们的结果。使用PrismaV8统计分析程序。在PDAC患者的血浆和微阵列组织中发现了四种DEmiRNA。来自患有PDAC的患者的血清样品用于验证它们在GEO数据库中的过表达。我们发现了一组新的miR-222-3p和miR-221-3p两种miRNA可用于诊断PDAC，当miR-221-3p和miR-222-3p过表达时，生存率下降。因此,miR-222-3p和miR-221-3p可用作墨西哥患者PDAC诊断和生存的非侵入性指标。
