PDF(Pubmed)
Abstract：
Monitoring the microenvironment within specific cellular regions is crucial for a comprehensive understanding of life events. Fluorescent probes working in different ranges of pH regions have been developed for the local imaging of different pH environments. Especially, rhodamine-based fluorescent pH probes have been of great interest due to their ON/OFF fluorescence depending on the spirolactam ring\'s opening/closure. By introducing the N-alkyl-hydroxamic acid instead of the alkyl amines in the spirolactam of rhodamine, we were able to tune the pH range where the ring opening and closing of the spirolactam occurs. This six-membered cyclic hydroxamate spirolactam ring of rhodamine B proved to be highly fluorescent in acidic pH environments. In addition, we could monitor pH changes of lysosomes in live cells and zebrafish.
摘要：
监测特定细胞区域内的微环境对于全面了解生活事件至关重要。已经开发了在不同pH区域范围内工作的荧光探针，用于不同pH环境的局部成像。尤其是,基于罗丹明的荧光pH探针由于其开/关荧光取决于螺内酰胺环的打开/关闭而引起了极大的兴趣。通过在罗丹明的螺内酰胺中引入N-烷基-异羟肟酸代替烷基胺，我们能够调节螺环内酰胺开环和闭环发生的pH范围。罗丹明B的这种六元环状异羟肟酸螺内酰胺环被证明在酸性pH环境中具有高度荧光性。此外,我们可以监测活细胞和斑马鱼中溶酶体的pH值变化。
