PDF(Pubmed)
Abstract：
Light plays a dominant role in the biosynthesis and accumulation of photosynthetic products. However, the metabolism and translocation of photosynthetic products in plants under different light spectra remain elusive. In this study, tomato (Solanum lycopersicum L.) seedlings were treated with different light spectra delivered by light-emitting diodes (LEDs) with the same photosynthetic photon flux density at 300 μmol m-2 s-1, including monochromatic red (660 nm, R), blue (450 nm, B), sun-like white (W, 380-780 nm), or a combination of R and B lights (R:B = 1:1, RB). Compared with W, the biomass distribution ratio for leaves under R, B, and RB decreased by 5.01-9.53%, while the ratio for stems and roots increased by 3.71-6.92% and 0.14-2.81%, respectively. The photosynthetic carbon distribution expressed as 13C enrichment was higher in stems and roots under RB and R, while B led to more 13C transported from leaves and enriched in stems when compared with W. Meanwhile, RB led to significant increases in the activities of phosphate synthase (SPS), sucrose synthase (SS), vacuolar acid invertase (VI), and neutral invertase (NI). The R was more efficient in increasing the activity of SPS and SS, while B was more effective in promoting the activity of VI and NI. The transcript levels of SPS, SS3, NI6, and VI were upregulated under R, B, and RB. However, the transcript patterns of SPS, SS3, NI6, and VI were not consistent with the changes in their encoded enzymes, especially the transcript patterns of SPS and SS3. Our study suggests that the red- and blue-light-induced long-distance and short-distance transport of photosynthetic products in plants, respectively, might result from different regulation of sucrose-metabolizing enzymes from transcriptional and post-transcriptional levels.
摘要：
光在光合产物的生物合成和积累中起着主导作用。然而,植物在不同光谱下的光合产物的代谢和转运仍然难以捉摸。在这项研究中,番茄（SolanumlycopersicumL.）幼苗用发光二极管（LED）提供的不同光谱处理，在300μmolm-2s-1下具有相同的光合光子通量密度，包括单色红色（660nm，R),蓝色(450nm,B),太阳般的白色（W，380-780nm），或R和B光的组合(R:B=1:1,RB)。与W相比，R下叶片的生物量分配比，B,RB下降5.01-9.53%，茎和根的比例分别增加了3.71-6.92%和0.14-2.81%，分别。在RB和R下，以13C富集表示的光合碳分布在茎和根中较高，而与W相比，B导致更多的13C从叶片运输并富含茎。同时，RB导致磷酸盐合酶（SPS）活性的显着增加，蔗糖合酶（SS），液泡酸性转化酶（VI），和中性转化酶(NI)。R在增加SPS和SS的活性方面更有效，而B在促进VI和NI的活性方面更有效。SPS的转录水平，SS3，NI6和VI在R下上调，B,和RB。然而,SPS的转录模式，SS3、NI6和VI与其编码酶的变化不一致，特别是SPS和SS3的转录模式。我们的研究表明，红光和蓝光诱导植物中光合产物的长距离和短距离运输，分别,可能是由于转录和转录后水平对蔗糖代谢酶的不同调节所致。
