PDF(Pubmed)
Abstract：
Pancreatic ductal adenocarcinoma (PDAC) represents the most frequent pancreatic malignancy, with stromal and epithelial heterogeneity reflected in outcome variability. Therefore, a molecular classification is promoted based on the validation of new diagnostic and prognostic markers. Galectin-8 (Gal8) has been pointed out as a prognostic factor for survival in several types of tumors. Due to limited existing data on PDAC, our study aimed to evaluate the Gal8 profile in PDAC alongside its prognostic status. A total of 87 cases of PDAC were immunohistochemically investigated, and Gal8 immunoexpression was qualitatively and semi-quantitatively assessed and correlated with classical clinicopathological parameters and survival. Gal8 immunoexpression was identified to be mostly nuclear and cytoplasmic, followed by exclusively cytoplasmic and exclusively nuclear. A statistical analysis between Gal8 profiles defined by negative, low, or high scores and clinicopathological characteristics showed significant differences in tumor size, pN stage, and lympho-vascular invasion. Although a Cox regression analysis did not support the prognostic status of Gal8, and we did not confirm its relationship with OS, our results show that exclusively nuclear labeling was associated with an increased mean OS compared with cytoplasmic and nuclear labeling (29.37 vs. 17.93 months). To the best of our knowledge, this is the first study to report a detailed pattern of Gal8 immunostaining in PDAC and to correlate this pattern with clinicopathological characteristics and survival. Our results show that Gal8 immunoexpression is associated with a more aggressive phenotype, thus opening perspectives for larger studies to validate Gal8 as a prognostic factor.
摘要：
胰腺导管腺癌（PDAC）是最常见的胰腺恶性肿瘤，结果变异性反映了基质和上皮的异质性。因此,基于新的诊断和预后标志物的验证,可促进分子分类.半乳糖凝集素-8(Gal8)已被指出为在几种类型的肿瘤中存活的预后因子。由于PDAC上的现有数据有限，我们的研究旨在评估PDAC的Gal8概况及其预后状态.对87例PDAC进行了免疫组化检查，对Gal8免疫表达进行定性和半定量评估，并与经典临床病理参数和生存率相关。Gal8免疫表达被鉴定为主要是细胞核和细胞质，其次是专门的细胞质和专门的核。由阴性定义的Gal8配置文件之间的统计分析，低,或高分和临床病理特征显示肿瘤大小有显著差异，pN阶段，和淋巴管浸润.尽管Cox回归分析不支持Gal8的预后状态，并且我们没有证实其与OS的关系，我们的结果表明，与细胞质和核标记相比，仅核标记与平均OS增加相关(29.37vs.17.93个月）。据我们所知,这是第一项报道PDAC中Gal8免疫染色的详细模式,并将该模式与临床病理特征和生存率相关联的研究.我们的结果表明，Gal8免疫表达与更具侵略性的表型相关，从而为验证Gal8作为预后因子的大型研究打开了视角.
