PDF(Pubmed)
Abstract：
Oxidative stress contributes to impairment of skin health, the wound healing process, and pathologies such as psoriasis or skin cancer. Five Polynesian medicinal plants, among the most traditionally used for skin care (pimples, wounds, burns, dermatoses) are studied herein for their antioxidant properties: Calophyllum inophyllum, Gardenia taitensis, Curcuma longa, Cordia subcordata, and Ficus prolixa. Plant extracts were submitted to in vitro bioassays related to antioxidant properties and their bioactive constituents were identified by a metabolomic analytical approach. High performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) analysis was performed leading to the characterization of 61 metabolites. Compounds annotated for F. prolixa and C. subcordata extracts were reported for the first time. Antioxidant properties were evaluated by total phenolic content (TPC), free radical scavenging DPPH (1,1-diphenyl-2-picryl-hydrazyl), and Ferric Reducing Antioxidant Power activity (FRAP) assays. F. prolixa extract was the most active one and showed antioxidant intracellular activity on keratinocytes by Anti Oxydant Power 1 assay. Online HPLC-DPPH allowed the identification of phenolic bioactive compounds such as quercetin-O-rhamnoside, rosmarinic acid, chlorogenic acid, procyanidins, epicatechin, 5-O-caffeoylshikimic acid, and curcumin as being responsible for the scavenging properties of these plant extracts. These results highlight the potential of F. prolixa aerial roots as a source of antioxidants for skin care applications.
摘要：
氧化应激会损害皮肤健康，伤口愈合过程，和病理如牛皮癣或皮肤癌。五种波利尼西亚药用植物，在最传统的皮肤护理中(丘疹，伤口,烧伤,皮肤病）在本文中研究了它们的抗氧化特性：Calophyluminophylum，栀子,姜黄,Cordiasubcordata,还有FicusProlixa.对植物提取物进行了与抗氧化特性相关的体外生物测定，并通过代谢组学分析方法鉴定了其生物活性成分。进行具有串联质谱的高效液相色谱(HPLC-MS/MS)分析,从而表征61种代谢物。对F.prolixa和C.subcordata提取物的注释为首次报道的化合物。通过总酚含量（TPC）评估抗氧化性能，自由基清除DPPH（1,1-二苯基-2-吡啶-肼基），和铁还原抗氧化能力活性(FRAP)测定。F.prolixa提取物是最有活性的一种，通过抗氧化能力1测定显示对角质形成细胞的抗氧化细胞活性。在线HPLC-DPPH可以鉴定酚类生物活性化合物，例如槲皮素-O-鼠李糖苷，迷迭香酸,绿原酸,原花青素,表儿茶素,5-O-咖啡酰基莽草酸，和姜黄素负责这些植物提取物的清除特性。这些结果突出了F.prolixa气生根作为皮肤护理应用的抗氧化剂来源的潜力。
