PDF(Pubmed)
Abstract：
Brain insulin resistance is linked to metabolic syndrome (MetS). A low-carbohydrate, high-fat (LCHF) diet has been proposed to have a protective effect. Therefore, this study aimed to investigate the brain insulin resistance markers in a rat animal model of MetS and the protective effects of the LCHF diet. Four groups of male rats (10/group) were created. Group I (Control) was fed a regular diet. Groups II-IV were injected with dexamethasone (DEX) to induce MetS. Group II received DEX with a regular diet. Group III (DEX + LCHF) rates were fed a low-carbohydrate, high-fat diet, while Group IV (DEX + HCLF) rats were fed a high-carbohydrate, low-fat (HCLF) diet. At the end of the four-week experiment, HOMA-IR was calculated. Moreover, cerebral gene expression analysis of S-100B, BDNF, TNF-α, IGF-1, IGF-1 R, IGFBP-2, IGFBP-5, Bax, Bcl-2, and caspase-3 was carried out. In the DEX group, rats showed a significant increase in the HOMA-IR and a decrease in the gene expression of IGF-1, IGF-1 R, IGFBP-2, IGFBP-5, BDNF, and Bcl2, with a concomitant rise in S100B, TNF-α, Bax, and caspase-3. The LCHF diet group showed a significantly opposite effect on all parameters. In conclusion, MetS is associated with dysregulated cerebral gene expression of BDNF, S100B, and TNF-α and disturbed IGF-1 signaling, with increased apoptosis and neuroinflammation. Moreover, the LCHF diet showed a protective effect, as evidenced by preservation of the investigated biochemical and molecular parameters.
摘要：
脑胰岛素抵抗与代谢综合征（MetS）有关。低碳水化合物，高脂肪（LCHF）饮食已被提出具有保护作用。因此,本研究旨在探讨MetS大鼠模型的脑胰岛素抵抗标志物及LCHF饮食的保护作用。四组雄性年夜鼠（10只/组）造就。第I组(对照)饲喂常规饮食。II-IV组注射地塞米松(DEX)诱导MetS。第二组接受定期饮食的DEX。第III组（DEX+LCHF）的比率被喂食低碳水化合物，高脂肪饮食,而IV组（DEX+HCLF）大鼠被喂食高碳水化合物，低脂（HCLF）饮食。在为期四周的实验结束时，计算HOMA-IR。此外，脑S-100B基因表达分析,BDNF,TNF-α,IGF-1,IGF-1R,IGFBP-2,IGFBP-5,Bax,进行Bcl-2和caspase-3。在DEX组中,大鼠的HOMA-IR显著增加,IGF-1,IGF-1R,IGFBP-2,IGFBP-5,BDNF,和Bcl2，伴随着S100B的上升，TNF-α,Bax,和caspase-3。LCHF饮食组对所有参数显示出明显相反的效果。总之,MetS与BDNF的大脑基因表达失调有关，S100B,和TNF-α和受干扰的IGF-1信号，细胞凋亡和神经炎症增加。此外，LCHF饮食显示出保护作用，所研究的生化和分子参数的保存证明了这一点。
