Abstract：
OBJECTIVE: To elucidate the effect of Jiaweidaochi powder on the Th1/Th2 ratio in rats with recurrent aphthous ulcers (RAU).
METHODS: 40 Sprague-Dawley rats were included in this study, randomly divided into a control group, a model group, and three treatment groups (0.5 g/ ml, 1 g/ml, 2 g/ml Jiaweidaochi powder). The RAU model of rats was established by autoantigen injection. The effects of Jiaweidaochi powder on the expression of INFG, IL-4, TBX21, and GATA3 mRNA were detected by real-time PCR. The expression of IFN-γ, IL-4, and IFN-γ/IL-4 proteins in oral ulcer tissue and serum of rats were analyzed by Western blot and ELISA, respectively. The proportion of Th1 cells and Th2 cells in RAU rats was analyzed by flow cytometry.
RESULTS: Jiaweidaochi powder reduced the number, diameter, and duration of oral ulcers in RAU rats. Real-time PCR showed that middle and high-dose Jiaweidaochi powder decreased the expression of INFG TBX21 mRNA and increased the expression of IL-4 and GATA3 mRNA in the oral tissue of RAU rats. ELISA and western blot confirmed that the expression of IFN-γ protein was significantly decreased, and the level of IL-4 protein was increased both in oral tissue and serum of RAU rats treated with middle or high doses of Jiaweidaochi powder. Flow cytometry found that the Jiaweidaochi powder decreased the proportion of Th1 cells and increased the proportion of Th2 cells in RAU rats.
CONCLUSIONS: This study found that Jiaweidaochi powder promoted the balance of Th1/Th2 in RAU rats, contributing to the healing of RAU.
METHODS: 40 Sprague-Dawley rats were included in this study, randomly divided into a control group, a model group, and three treatment groups (0.5 g/ ml, 1 g/ml, 2 g/ml Jiaweidaochi powder). The RAU model of rats was established by autoantigen injection. The effects of Jiaweidaochi powder on the expression of INFG, IL-4, TBX21, and GATA3 mRNA were detected by real-time PCR. The expression of IFN-γ, IL-4, and IFN-γ/IL-4 proteins in oral ulcer tissue and serum of rats were analyzed by Western blot and ELISA, respectively. The proportion of Th1 cells and Th2 cells in RAU rats was analyzed by flow cytometry.
RESULTS: Jiaweidaochi powder reduced the number, diameter, and duration of oral ulcers in RAU rats. Real-time PCR showed that middle and high-dose Jiaweidaochi powder decreased the expression of INFG TBX21 mRNA and increased the expression of IL-4 and GATA3 mRNA in the oral tissue of RAU rats. ELISA and western blot confirmed that the expression of IFN-γ protein was significantly decreased, and the level of IL-4 protein was increased both in oral tissue and serum of RAU rats treated with middle or high doses of Jiaweidaochi powder. Flow cytometry found that the Jiaweidaochi powder decreased the proportion of Th1 cells and increased the proportion of Th2 cells in RAU rats.
CONCLUSIONS: This study found that Jiaweidaochi powder promoted the balance of Th1/Th2 in RAU rats, contributing to the healing of RAU.
摘要：
目的：阐明加味导赤散对复发性阿弗他溃疡(RAU)大鼠Th1/Th2比值的影响。
方法：本研究包括40只Sprague-Dawley大鼠，随机分为对照组,一个模型组，和三个治疗组（0.5g/ml，1g/ml，2μg/ml加味道赤粉)。通过自身抗原注射建立大鼠RAU模型。加味泡粉对INFG表达的影响,通过实时PCR检测IL-4、TBX21和GATA3mRNA。IFN-γ的表达,采用Westernblot和ELISA方法分析大鼠口腔溃疡组织和血清中的IL-4和IFN-γ/IL-4蛋白,分别。流式细胞术分析RAU大鼠Th1细胞和Th2细胞的比例。
结果：加味泡粉减少了数量，直径,RAU大鼠口腔溃疡的持续时间。Real-timePCR显示，中、高剂量加味泡粉可降低RAU大鼠口腔组织中INFGTBX21mRNA的表达，增加IL-4和GATA3mRNA的表达。ELISA和westernblot证实IFN-γ蛋白表达显著降低,中、高剂量加味导赤散治疗RAU大鼠口腔组织和血清中IL-4蛋白水平均升高。流式细胞仪检测发现加味导胆散能降低RAU大鼠Th1细胞比例,升高Th2细胞比例。
结论：本研究发现加味导池散促进RAU大鼠Th1/Th2平衡，有助于RAU的愈合。
方法：本研究包括40只Sprague-Dawley大鼠，随机分为对照组,一个模型组，和三个治疗组（0.5g/ml，1g/ml，2μg/ml加味道赤粉)。通过自身抗原注射建立大鼠RAU模型。加味泡粉对INFG表达的影响,通过实时PCR检测IL-4、TBX21和GATA3mRNA。IFN-γ的表达,采用Westernblot和ELISA方法分析大鼠口腔溃疡组织和血清中的IL-4和IFN-γ/IL-4蛋白,分别。流式细胞术分析RAU大鼠Th1细胞和Th2细胞的比例。
结果：加味泡粉减少了数量，直径,RAU大鼠口腔溃疡的持续时间。Real-timePCR显示，中、高剂量加味泡粉可降低RAU大鼠口腔组织中INFGTBX21mRNA的表达，增加IL-4和GATA3mRNA的表达。ELISA和westernblot证实IFN-γ蛋白表达显著降低,中、高剂量加味导赤散治疗RAU大鼠口腔组织和血清中IL-4蛋白水平均升高。流式细胞仪检测发现加味导胆散能降低RAU大鼠Th1细胞比例,升高Th2细胞比例。
结论：本研究发现加味导池散促进RAU大鼠Th1/Th2平衡，有助于RAU的愈合。
