PDF(Pubmed)
Abstract：
Rumen flukes are geographically widespread trematodes affecting wild and domestic ruminants. The juvenile forms, which are found in the small intestine, are more pathogenic compared to the adults. Severe diarrhoea and weight loss are the major clinical signs, and the disease might be fatal in severely infested individuals. In the last decade, paramphistomosis has been described as an emerging parasitic disease in Europe. This study aimed to identify the rumen fluke species in wild ruminants from western Romania. Fifty-two pre-stomachs obtained from roe deer (Capreolus capreolus) that were hunted on 14 hunting grounds from Timiș and Arad counties were examined for the presence of paramphistomes. Three (9.09%) out of 33 samples were positive in Timiș County, and one (5.26%) out of 19 samples was positive in Arad County. Subsequent PCR testing revealed that three samples were positive for Calicophoron daubneyi and one for Paramphistomum leydeni. The presence of C. daubneyi and P. leydeni in roe deer has not been previously reported in Romania. Two Paramphistomum species-C. daubneyi and P. leydeni-were revealed as the main species of rumen flukes in roe deer from forests in Romania.
摘要：
瘤胃吸虫是影响野生和家养反刍动物的地理上广泛分布的吸虫。少年形式，在小肠中发现，与成年人相比更具致病性。严重腹泻和体重减轻是主要的临床症状，这种疾病在严重感染的个体中可能是致命的。在过去的十年里，在欧洲，杂种病已被描述为一种新兴的寄生虫病。本研究旨在鉴定罗马尼亚西部野生反刍动物的瘤胃吸虫种类。检查了在蒂米斯和阿拉德县的14个猎场上猎捕的从ro（Capreoluscapreolus）获得的52个胃中是否存在两栖动物。在提米县，33个样本中有3个(9.09%)呈阳性，19份样本中有1份(5.26%)在阿拉德县呈阳性。随后的PCR检测显示，三份样品对Calicophorondaubneyi呈阳性，一份对Paramistostomumleydeni呈阳性。罗马尼亚以前没有报道过在the中存在C.daubneyi和P.leydeni。两种两栖动物-C。daubneyi和P.leydeni-被发现是罗马尼亚森林中ro的瘤胃吸虫的主要物种。
