PDF(Pubmed)
Abstract：
Incisional hernia is a frequent complication after abdominal surgery. A previous study on rats evaluated the use of a Pectin-Honey Hydrogel (PHH)-coated polypropylene (PP) mesh for the healing of acute hernias. However, there are no studies investigating the use of PHH in association with PP mesh in chronic contaminated hernia. The aims of this study are to assess the effectiveness of PHH in promoting abdominal hernia repaired with PP mesh and in counteracting infection. Twenty Sprague Dawley male rats were enrolled and a full thickness defect was made in the abdominal wall. The defect was repaired after 28 days using a PP mesh, and a culture medium (Tryptone Soy Broth, Oxoid) was spread onto the mesh to contaminate wounds in both groups. The rats were randomly assigned to a treated or untreated group. In the treated group, a PHH was applied on the mesh before skin closure. At euthanasia-14 days after surgery-macroscopical, microbiological and histopathological evaluations were performed, with a score attributed for signs of inflammation. An immunohistochemical investigation against COX-2 was also performed. Adhesions were more severe (p = 0.0014) and extended (p = 0.0021) in the untreated group. Bacteriological results were not significantly different between groups. Both groups showed moderate to severe values (score > 2) in terms of reparative and inflammatory reactions at histopathological levels. The use of PHH in association with PP mesh could reduce adhesion formation, extension and severity compared to PP mesh alone. No differences in terms of wound healing, contamination and grade of inflammation were reported between groups.
摘要：
切口疝是腹部手术后常见的并发症。先前对大鼠的研究评估了使用果胶-蜂蜜水凝胶（PHH）涂覆的聚丙烯（PP）网片治疗急性疝气。然而,没有研究调查PHH与PP网片在慢性污染疝中的应用.这项研究的目的是评估PHH在促进用PP网片修复腹部疝和抵抗感染方面的有效性。招募20只SpragueDawley雄性大鼠，并在腹壁形成全层缺损。28天后使用PP网修复缺陷，和培养基(胰蛋白酮大豆肉汤，将Oxoid）扩散到网状物上以污染两组的伤口。将大鼠随机分配到治疗组或未治疗组。在治疗组中，在皮肤闭合之前,将PHH施加在网网上。在安乐死-手术后14天-宏观，进行了微生物学和组织病理学评估，评分归因于炎症的迹象。还进行了针对COX-2的免疫组织化学研究。在未治疗组中,粘连更严重(p=0.0014)和延长(p=0.0021)。细菌学结果在组间没有显著差异。两组在组织病理学水平的修复性和炎性反应方面均显示中度至重度值(评分>2)。PHH与PP网眼联合使用可以减少粘附形成，与单独的PP网格相比，延伸和严重程度。伤口愈合方面没有差异，报告了组间的污染和炎症等级.
