PDF(Pubmed)
Abstract：
Biological augmentation of bony defects in weight-bearing areas of both the acetabulum and the femur remains challenging. The calcium-silicate-based ceramic Baghdadite is a very interesting material to be used in the field of revision total hip arthroplasty for the treatment of bony defects in weight-bearing and non-weight-bearing areas alike. The aim of this study was to investigate the biocompatibility of Baghdadite utilizing an osteoblast-like, human osteosarcoma cell line (MG-63) and the human monocytic leukemia-derived cell line (THP-1). THP-1-derived macrophages and MG-63 were indirectly exposed to Baghdadite for 7 days using a transwell system. Viability was assessed with MTT assay and pH analysis. To investigate proliferation rate, both cell lines were labelled using CFSE and flow cytometrically analyzed. ELISA was used to measure the secretion of IL-1ß, IL-6 and TNFα. The investigation of viability, while showing a slight difference in optical density for the MTT assays in MG-63 cells, did not present a meaningful difference between groups for both cell lines. The comparison of pH and the proportion of living cells between groups did not present with a significant difference for both THP-1 and MG-63. Baghdadite did not have a relevant impact on the proliferation rate of the investigated cell lines. Mean fluorescence intensity was calculated between groups with no significant difference. Baghdadite exerted a proinflammatory effect, which could be seen in an upregulated production of TNFα in macrophages. Production of IL-1ß and IL-6 was not statistically significant, but the IL-6 ELISA showed a trend to an upregulated production as well. A similar effect on MG-63 was not observed. No relevant cytotoxicity of Baghdadite ceramics was encountered. Baghdadite ceramics exhibit a proinflammatory potential by significantly increasing the secretion of TNFα in THP-1-derived macrophages. Whether this proinflammatory potential results in a clinically relevant effect on osteointegration is unclear and requires further investigation. Baghdadite ceramics provide an interesting alternative to conventional bone substitutes and should be further investigated in a biomechanical and in vivo setting.
摘要：
髋臼和股骨负重区域骨缺损的生物学增强仍然具有挑战性。硅酸钙基陶瓷Baghdadite是一种非常有趣的材料，可用于翻修全髋关节置换术领域，用于治疗负重和非负重区域的骨缺损。这项研究的目的是研究Baghdadite利用成骨细胞样的生物相容性，人骨肉瘤细胞系（MG-63）和人单核细胞白血病衍生细胞系（THP-1）。使用transwell系统将THP-1衍生的巨噬细胞和MG-63间接暴露于Baghdadite7天。用MTT测定和pH分析评估活力。为了研究增殖率，两种细胞系均使用CFSE标记,并进行流式细胞计数分析.ELISA用于测量IL-1β的分泌，IL-6和TNFα。生存能力的调查，虽然在MG-63细胞中MTT测定的光密度略有差异，对于两种细胞系，组间没有有意义的差异。对于THP-1和MG-63，组间pH和活细胞比例的比较没有显着差异。Baghdadite对所研究细胞系的增殖率没有相关影响。计算组间的平均荧光强度,没有显著差异。Baghdadite发挥了促炎作用，这可以在巨噬细胞中TNFα的上调产生中看到。IL-1β和IL-6的产生没有统计学意义，但IL-6ELISA也显示了产量上调的趋势。未观察到对MG-63的类似作用。没有遇到Baghdadite陶瓷的相关细胞毒性。Baghdadite陶瓷通过显着增加THP-1衍生的巨噬细胞中TNFα的分泌而表现出促炎潜力。这种促炎潜能是否导致对骨整合的临床相关作用尚不清楚，需要进一步研究。Baghdadite陶瓷为常规骨替代品提供了一种有趣的替代方法，应在生物力学和体内环境中进一步研究。
