Abstract：
Current study aims to investigate the biological function of circular RNA (circRNA, circ_0000337) in cervical cancer (CC). Bioinformatic analyses were used to predict targets for circ_0000337 and miR-155-5p, and analyze the gene expression differences between cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) tissues and normal tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were applied to assess mRNA and protein expressions of circ_0000337, microRNA-155-5p (miR-155-5p) and member RAS oncogene family (RAB3B), respectively. Following the establishment of gain/loss-of-function models, CCK-8 was performed to evaluate cell proliferation. Bioinformatics analysis, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) were used to identify the interaction in circ_0000337, miR-155-5p, and RAB3B. Circ_0000337 and RAB3B were upregulated, while miR-155-5p was downregulated in CC tissues and cell lines. circ_0000337 overexpression promoted cell proliferation, circ_0000337 knock down inhibited cell proliferation by sponging miR-155-5p. RAB3B was a target of miR-155-5p which was positively regulated by circ_0000337. In the collected CC tissues, there was a negative correlation between miR-155-5p and circ_0000337 or RAB3B, and a positive correlation between circ_0000337 and RAB3B. miR-155-5p was positively, while RAB3B was negatively correlated with OS in patients with CC, and they were negatively correlated. In conclusion, circ_0000337 upregulates RAB3B by sponging miR-155-5p to promote CC cell proliferation.
摘要：
目前的研究旨在探讨环状RNA(circularRNA,circRNA,circ_0000337)在宫颈癌(CC)中。生物信息学分析用于预测circ_0000337和miR-155-5p的靶标，并分析宫颈鳞癌和宫颈腺癌(CESC)组织与正常组织的基因表达差异。定量实时聚合酶链反应（qRT-PCR）和Westernblot用于评估circ_0000337，microRNA-155-5p（miR-155-5p）和RAS癌基因家族成员（RAB3B）的mRNA和蛋白表达。分别。在建立功能得失模型之后，进行CCK-8以评估细胞增殖。生物信息学分析，双荧光素酶报告基因测定和RNA免疫沉淀（RIP）用于鉴定circ_0000337，miR-155-5p，RAB3BCirc_0000337和RAB3B上调，而miR-155-5p在CC组织和细胞系中下调。circ_0000337过表达促进细胞增殖，circ_0000337敲低通过生成miR-155-5p抑制细胞增殖。RAB3B是miR-155-5p的靶标,其被circ_0000337正调控。在收集的CC组织中，miR-155-5p与circ_0000337或RAB3B呈负相关,circ_0000337与RAB3B呈正相关。miR-155-5p呈阳性，而CC患者的RAB3B与OS呈负相关，它们呈负相关。总之,circ_0000337通过膨胀miR-155-5p来上调RAB3B以促进CC细胞增殖。
