Abstract：
OBJECTIVE: Vasovasostomy is used to correct vas deferens (VD) transections encountered during surgery or to reverse sterilization vasectomies. Achieving vasal patency is the primary goal and the success is assessed on various factors including VD patency, flow rates, and pregnancy rates. While preserving vas motility is not a major concern in surgical practice, it is worth noting that VD has peristaltic activity which plays crucial role during ejaculation. Any disruption in its motility could potentially lead to negative outcomes in the future. We conducted an experimental study to assess vas motility changes following vasovasostomy.
METHODS: The study was approved by Gazi University, Animals Ethic Committee. Twenty-four rats were allocated to four groups. Left-sided VD was harvested in control group (Gr1). The rest of the animals were subjected to transection of VD. Gr2 and 3 underwent microscopic and macroscopic anastomosis, respectively, while Gr4 underwent vasal approximation. After 12 weeks, all left-sided VD were resected, electrical field stimulation (EFS) and exogenous drugs were applied to induce contractions. Statistical analyses were performed and p value < 0.05 was regarded as statistically significant.
RESULTS: The first and second phases of EFS-induced contractile responses(CR) increased for Gr3 and decreased for Gr4 at submaximal and maximal frequencies. An increase only at maximal frequency for second phase EFS-induced CR was encountered for Gr2. α-β-methylene-ATP-induced CR decreased for Gr3 and 4. Noradrenaline-induced CR increased for Gr2, and 3 and decreased for Gr4.
CONCLUSIONS: The results suggest that vasovasostomy performed using a surgical technique that minimizes disruption or damage to VD may have a favorable impact on motility.
METHODS: The study was approved by Gazi University, Animals Ethic Committee. Twenty-four rats were allocated to four groups. Left-sided VD was harvested in control group (Gr1). The rest of the animals were subjected to transection of VD. Gr2 and 3 underwent microscopic and macroscopic anastomosis, respectively, while Gr4 underwent vasal approximation. After 12 weeks, all left-sided VD were resected, electrical field stimulation (EFS) and exogenous drugs were applied to induce contractions. Statistical analyses were performed and p value < 0.05 was regarded as statistically significant.
RESULTS: The first and second phases of EFS-induced contractile responses(CR) increased for Gr3 and decreased for Gr4 at submaximal and maximal frequencies. An increase only at maximal frequency for second phase EFS-induced CR was encountered for Gr2. α-β-methylene-ATP-induced CR decreased for Gr3 and 4. Noradrenaline-induced CR increased for Gr2, and 3 and decreased for Gr4.
CONCLUSIONS: The results suggest that vasovasostomy performed using a surgical technique that minimizes disruption or damage to VD may have a favorable impact on motility.
摘要：
目的：血管造口术用于纠正手术中遇到的输精管（VD）横切或逆转灭菌输精管切除术。实现血管通畅是首要目标，其成功取决于各种因素，包括VD通畅性。流速,和怀孕率。虽然在手术实践中保留血管运动性并不是一个主要问题，值得注意的是，VD具有蠕动活性，在射精过程中起着至关重要的作用。其运动能力的任何中断都可能导致未来的负面结果。我们进行了一项实验研究，以评估血管造口术后的血管运动性变化。
方法：这项研究得到了加济大学的批准，动物伦理委员会。将24只大鼠分为四组。对照组(Gr1)收获左侧VD。对其余动物进行VD横切。Gr2和3进行了显微和宏观吻合，分别,而Gr4进行了血管逼近。12周后,所有左侧VD均被切除，应用电场刺激（EFS）和外源性药物诱导收缩。进行统计学分析,p值<0.05被认为是统计学上显著的。
结果：在亚最大和最大频率下，EFS诱导的收缩反应（CR）的第一阶段和第二阶段对于Gr3增加，对于Gr4降低。Gr2仅在第二阶段EFS诱导的CR的最大频率下增加。对于Gr3和Gr4,α-β-亚甲基-ATP诱导的CR降低。去甲肾上腺素诱导的CR对于Gr2和3增加，对于Gr4降低。
结论：结果表明，使用外科技术进行的血管血管造口术可以最大程度地减少VD的破坏或损害，可能对运动产生有利的影响。
方法：这项研究得到了加济大学的批准，动物伦理委员会。将24只大鼠分为四组。对照组(Gr1)收获左侧VD。对其余动物进行VD横切。Gr2和3进行了显微和宏观吻合，分别,而Gr4进行了血管逼近。12周后,所有左侧VD均被切除，应用电场刺激（EFS）和外源性药物诱导收缩。进行统计学分析,p值<0.05被认为是统计学上显著的。
结果：在亚最大和最大频率下，EFS诱导的收缩反应（CR）的第一阶段和第二阶段对于Gr3增加，对于Gr4降低。Gr2仅在第二阶段EFS诱导的CR的最大频率下增加。对于Gr3和Gr4,α-β-亚甲基-ATP诱导的CR降低。去甲肾上腺素诱导的CR对于Gr2和3增加，对于Gr4降低。
结论：结果表明，使用外科技术进行的血管血管造口术可以最大程度地减少VD的破坏或损害，可能对运动产生有利的影响。
