Abstract:
Chickpea (Cicer arietinum L.) is one of the most important crops worldwide and a valuable nutritional source. The availability of data from different genotypes and populations is important for the comprehension of the biology and trait control of chickpea. Tissue from young leaves was collected from adult plants and sequenced using an Illumina HiSeq X platform, which provided sequencing data for a total of 169 individuals from two different populations. Furthermore, functional annotation was performed with BLAST2GO software in a candidate region for resistance to Ascochyta blight, a devastating disease that produces huge yield reductions if the growth conditions are optimal for the fungus. A total of 273 different genes in a region spanning ∼4.67 Mb in chromosome 4 were fully annotated. The raw DNA sequences and functional annotation data can be reused by the scientific community for the analysis of different agronomic traits of interest in chickpea.
摘要:
鹰嘴豆(CicerarietinumL.)是世界上最重要的农作物之一,是宝贵的营养来源。来自不同基因型和种群的数据的可用性对于理解鹰嘴豆的生物学和性状控制很重要。从成年植物中收集幼叶的组织,并使用IlluminaHiSeqX平台进行测序,它提供了来自两个不同群体的总共169名个体的测序数据。此外,用BLAST2GO软件在抗Ascochyta枯萎病的候选区域进行功能注释,一种毁灭性的疾病,如果真菌的生长条件是最佳的,会导致产量大幅下降。完整注释了4号染色体约4.67Mb区域中的273个不同基因。原始DNA序列和功能注释数据可被科学界重复使用,用于分析鹰嘴豆中感兴趣的不同农艺性状。