Abstract：
This review provides an overview of the latest developments in both experimental and simulation techniques used to assess the bending rigidity of lipid membranes. It places special emphasis on experimental methods that utilize model vesicles to manipulate lipid compositions and other experimental parameters to determine the bending rigidity of the membrane. It also describes two commonly used simulation methods for estimating bending rigidity. The impact of various factors on membrane bending rigidity is summarized, including cholesterol, lipids, salt concentration, surface charge, membrane phase state, peptides, proteins, and polyethylene glycol. These factors are shown to influence the bending rigidity, contributing to a better understanding of the biophysical properties of membranes and their role in biological processes. Furthermore, the review discusses future directions and potential advancements in this research field, highlighting areas where further investigation is required.
摘要：
这篇综述概述了用于评估脂质膜弯曲刚度的实验和模拟技术的最新进展。它特别强调利用模型囊泡来操纵脂质组成和其他实验参数以确定膜的弯曲刚度的实验方法。它还描述了两种常用的用于估计弯曲刚度的模拟方法。总结了各种因素对膜弯曲刚度的影响,包括胆固醇,脂质，盐浓度,表面电荷,膜相态,肽，蛋白质，和聚乙二醇。这些因素被证明会影响弯曲刚度，有助于更好地了解膜的生物物理特性及其在生物过程中的作用。此外，这篇综述讨论了这一研究领域的未来方向和潜在进展，强调需要进一步调查的领域。
