In order to examine the disparities in the structure and diversity of soil microbial communities across distinct vegetation types, we opted to utilize sample plots representing four specific vegetation types. These included a woodland with the dominant tree species Drypetes perreticulata, a woodland with the dominant tree species Horsfieldia hainanensis, a Zea mays farmland and a Citrus reticulata fields. Through the application of high-throughput sequencing, the 16S V3_V4 region of soil bacteria and the ITS region of fungi were sequenced in this experiment. Subsequently, a comparative analysis was conducted to explore and assess the structure and dissimilarities of soil bacterial and fungal communities of the four vegetation types were analyzed comparatively.
Our findings indicated that woodland soil exhibit a higher richness of microbial diversity compared to farmland soils. There were significant differences between woodland and farmland soil microbial community composition. However, all four dominant phyla of soil fungi were Ascomycota across the four vegetation types, but the bacterial dominant phyla were different in the two-farmland soil microbial communities with the highest similarity. Furthermore, we established a significant correlation between the nutrient content of different vegetation types and the relative abundance of soil microorganisms at both phyla and genus levels. This experiment serves as a crucial step towards unraveling the intricate relationships between plants, soil microbes, and soil, as well as understanding the underlying driving mechanism.
■为了研究不同植被类型土壤微生物群落结构和多样性的差异,我们选择利用代表四种特定植被类型的样地。其中包括具有优势树种的林地。具有优势树种海南岛Horsfieldia的林地,玉米农田和柑橘田地。通过高通量测序的应用,本实验对土壤细菌的16SV3_V4区和真菌的ITS区进行了测序。随后,进行了比较分析,以探索和评估四种植被类型的土壤细菌和真菌群落的结构和差异。
■我们的发现表明,与农田土壤相比,林地土壤表现出更高的微生物多样性丰富度。林地和农田土壤微生物群落组成存在显著差异。然而,在四种植被类型中,土壤真菌的所有四种优势门都是子囊菌,但两个农田土壤微生物群落中细菌优势门的相似性最高。此外,我们在门和属水平上建立了不同植被类型的养分含量与土壤微生物相对丰度之间的显着相关性。这个实验是解开植物之间复杂关系的关键一步,土壤微生物,土壤,以及了解潜在的驱动机制。