Abstract：
BACKGROUND: Neuroprotective agents are needed to reduce cerebral damage during surgical or neurointerventional procedures including stroke patients.
OBJECTIVE: To evaluate if thiopental can be used as a neuroprotective agent when injected intra-arterially in a transient ischemia model.
METHODS: In total, 24 rabbits were studied as four groups of six animals. Group 1 served as the control group. In group 2, transient ischemia was obtained by intracarotid administration of degradable starch microspheres (DSM). Group 3 was administered thiopental intra-arterially via the carotid artery. Group 4 (experimental group) received both thiopental and DSM intra-arterially. DSM and thiopental were administered through a microcatheter placed into the common carotid artery via the central ear artery access. After sacrifice, apoptotic cells in the cerebral tissues of the animals were evaluated in H&E and TUNEL stained slides.
RESULTS: There was a significant increase in the number of apoptotic glial or neuronal cells in group 2 compared to the control group and group 3. The mean number of both the apoptotic neuronal cells (6.8 ± 2.1 vs. 2.5 ± 1.3, P < 0.001) and the apoptotic glial cells (9.4 ± 3.1 vs. 4.6 ± 1.6, P < 0.001) were higher in group 2 compared to group 4. In addition, a higher level of neurological improvement was observed in group 4 compared to group 2 based on neurological assessment score.
CONCLUSIONS: The intra-arterial administration of thiopental has a protective effect on both glial and neuronal cells during temporary cerebral ischemia in low doses.
OBJECTIVE: To evaluate if thiopental can be used as a neuroprotective agent when injected intra-arterially in a transient ischemia model.
METHODS: In total, 24 rabbits were studied as four groups of six animals. Group 1 served as the control group. In group 2, transient ischemia was obtained by intracarotid administration of degradable starch microspheres (DSM). Group 3 was administered thiopental intra-arterially via the carotid artery. Group 4 (experimental group) received both thiopental and DSM intra-arterially. DSM and thiopental were administered through a microcatheter placed into the common carotid artery via the central ear artery access. After sacrifice, apoptotic cells in the cerebral tissues of the animals were evaluated in H&E and TUNEL stained slides.
RESULTS: There was a significant increase in the number of apoptotic glial or neuronal cells in group 2 compared to the control group and group 3. The mean number of both the apoptotic neuronal cells (6.8 ± 2.1 vs. 2.5 ± 1.3, P < 0.001) and the apoptotic glial cells (9.4 ± 3.1 vs. 4.6 ± 1.6, P < 0.001) were higher in group 2 compared to group 4. In addition, a higher level of neurological improvement was observed in group 4 compared to group 2 based on neurological assessment score.
CONCLUSIONS: The intra-arterial administration of thiopental has a protective effect on both glial and neuronal cells during temporary cerebral ischemia in low doses.
摘要：
背景：需要神经保护剂来减少包括中风患者在内的手术或神经介入治疗过程中的脑损伤。
目的：评估在短暂性缺血模型中动脉内注射硫喷妥钠是否可用作神经保护剂。
方法：总共，将24只兔子作为四组六只动物进行研究。第1组作为对照组。在第2组中，通过颈动脉内施用可降解淀粉微球（DSM）获得短暂性缺血。第3组通过颈动脉动脉内给予硫喷妥钠。第4组（实验组）动脉内接受硫喷妥钠和DSM。DSM和硫喷妥钠通过经中央耳动脉进入颈总动脉的微导管给药。牺牲之后,在H&E和TUNEL染色的载玻片中评估动物脑组织中的凋亡细胞。
结果：与对照组和第3组相比，第2组的凋亡神经胶质或神经元细胞数量显着增加。两个凋亡神经元细胞的平均数（6.8±2.1vs.2.5±1.3，P<0.001）和凋亡神经胶质细胞（9.4±3.1vs.第2组的4.6±1.6，P<0.001）高于第4组。此外,根据神经学评估评分,与第2组相比,第4组的神经学改善水平更高.
结论：低剂量的动脉内注射硫喷妥钠对暂时性脑缺血期间的神经胶质细胞和神经元细胞均具有保护作用。
目的：评估在短暂性缺血模型中动脉内注射硫喷妥钠是否可用作神经保护剂。
方法：总共，将24只兔子作为四组六只动物进行研究。第1组作为对照组。在第2组中，通过颈动脉内施用可降解淀粉微球（DSM）获得短暂性缺血。第3组通过颈动脉动脉内给予硫喷妥钠。第4组（实验组）动脉内接受硫喷妥钠和DSM。DSM和硫喷妥钠通过经中央耳动脉进入颈总动脉的微导管给药。牺牲之后,在H&E和TUNEL染色的载玻片中评估动物脑组织中的凋亡细胞。
结果：与对照组和第3组相比，第2组的凋亡神经胶质或神经元细胞数量显着增加。两个凋亡神经元细胞的平均数（6.8±2.1vs.2.5±1.3，P<0.001）和凋亡神经胶质细胞（9.4±3.1vs.第2组的4.6±1.6，P<0.001）高于第4组。此外,根据神经学评估评分,与第2组相比,第4组的神经学改善水平更高.
结论：低剂量的动脉内注射硫喷妥钠对暂时性脑缺血期间的神经胶质细胞和神经元细胞均具有保护作用。
